Identification of Antimonate Reducing Bacteria and Their Potential Metabolic Traits by the Combination of Stable Isotope Probing and Metagenomic-Pangenomic Analysis

2021 ◽  
Author(s):  
Weimin Sun ◽  
Xiaoxu Sun ◽  
Max M. Häggblom ◽  
Max Kolton ◽  
Ling Lan ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing ◽  
Metabolic Traits ◽  
Reducing Bacteria

scholarly journals Versatile cyanobacteria control the timing and extent of sulfide production in a Proterozoic analog microbial mat

2020 ◽  
Vol 14 (12) ◽  
pp. 3024-3037 ◽  
Author(s):  
Judith M. Klatt ◽  
Gonzalo V. Gomez-Saez ◽  
Steffi Meyer ◽  
Petra Pop Ristova ◽  
Pelin Yilmaz ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Organic Carbon ◽  
Stable Isotope Probing ◽  
Cyanobacterial Mats ◽  
Anoxygenic Photosynthesis ◽  
Sulfide Production ◽  
Sulfur Oxidizing ◽  
Benthic Ecosystems ◽  
Reducing Bacteria ◽  
Synergistic Relationship

Abstract Cyanobacterial mats were hotspots of biogeochemical cycling during the Precambrian. However, mechanisms that controlled O2 release by these ecosystems are poorly understood. In an analog to Proterozoic coastal ecosystems, the Frasassi sulfidic springs mats, we studied the regulation of oxygenic and sulfide-driven anoxygenic photosynthesis (OP and AP) in versatile cyanobacteria, and interactions with sulfur reducing bacteria (SRB). Using microsensors and stable isotope probing we found that dissolved organic carbon (DOC) released by OP fuels sulfide production, likely by a specialized SRB population. Increased sulfide fluxes were only stimulated after the cyanobacteria switched from AP to OP. O2 production triggered migration of large sulfur-oxidizing bacteria from the surface to underneath the cyanobacterial layer. The resultant sulfide shield tempered AP and allowed OP to occur for a longer duration over a diel cycle. The lack of cyanobacterial DOC supply to SRB during AP therefore maximized O2 export. This mechanism is unique to benthic ecosystems because transitions between metabolisms occur on the same time scale as solute transport to functionally distinct layers, with the rearrangement of the system by migration of microorganisms exaggerating the effect. Overall, cyanobacterial versatility disrupts the synergistic relationship between sulfide production and AP, and thus enhances diel O2 production.

scholarly journals Molecular Analysis of Arsenate-Reducing Bacteria within Cambodian Sediments following Amendment with Acetate

2006 ◽  
Vol 73 (4) ◽  
pp. 1041-1048 ◽  
Author(s):  
G. Lear ◽  
B. Song ◽  
A. G. Gault ◽  
D. A. Polya ◽  
J. R. Lloyd
Keyword(s):  
Drinking Water ◽  
Organic Matter ◽  
Stable Isotope ◽  
Bacterial Communities ◽  
Microbial Reduction ◽  
Stable Isotope Probing ◽  
Direct Link ◽  
Present Evidence ◽  
Reducing Bacteria ◽  
Arsenate Reducing Bacteria

ABSTRACT The health of millions is threatened by the use of groundwater contaminated with sediment-derived arsenic for drinking water and irrigation purposes in Southeast Asia. The microbial reduction of sorbed As(V) to the potentially more mobile As(III) has been implicated in release of arsenic into groundwater, but to date there have been few studies of the microorganisms that can mediate this transformation in aquifers. With the use of stable isotope probing of nucleic acids, we present evidence that the introduction of a proxy for organic matter (13C-labeled acetate) stimulated As(V) reduction in sediments collected from a Cambodian aquifer that hosts arsenic-rich groundwater. This was accompanied by an increase in the proportion of prokaryotes closely related to the dissimilatory As(V)-reducing bacteria Sulfurospirillum strain NP-4 and Desulfotomaculum auripigmentum. As(V) respiratory reductase genes (arrA) closely associated with those found in Sulfurospirillum barnesii and Geobacter uraniumreducens were also detected in active bacterial communities utilizing 13C-labeled acetate in microcosms. This study suggests a direct link between inputs of organic matter and the increased prevalence and activity of organisms which transform As(V) to the potentially more mobile and thus hazardous As(III) via dissimilatory As(V) reduction.

Untargeted Stable Isotope Probing of the Gut Microbiota Metabolome Using 13C-Labeled Dietary Fibers

2021 ◽  
Author(s):  
Pan Deng ◽  
Taylor Valentino ◽  
Michael D. Flythe ◽  
Hunter N. B. Moseley ◽  
Jacqueline R. Leachman ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Gut Microbiota ◽  
Stable Isotope Probing ◽  
Dietary Fibers
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