Ligand-Enhanced Electron Utilization for Trichloroethylene Degradation by ·OH during Sediment Oxygenation

Author(s):  
Wenjing Xie ◽  
Peng Zhang ◽  
Wenjuan Liao ◽  
Man Tong ◽  
Songhu Yuan
2005 ◽  
Vol 51 (6) ◽  
pp. 433-439 ◽  
Author(s):  
J A Humphries ◽  
A M.H Ashe ◽  
J A Smiley ◽  
C G Johnston

Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a possible remedial strategy. Incubations of14C-TCE-spiked groundwater amended with phenol showed increased TCE mineralization compared with unamended groundwater. Community structure was determined using DNA directly extracted from groundwater samples. This DNA was then analyzed by amplified ribosomal DNA restriction analysis. Unique restriction fragment length polymorphisms defined operational taxonomic units that were sequenced to determine phylogeny. DNA sequence data indicated that known TCE-degrading bacteria including relatives of Variovorax and Burkholderia were present in site water. Diversity of the amplified microbial rDNA clone library was lower in phenol-amended communities than in unamended groundwater (i.e., having Shannon–Weaver diversity indices of 2.0 and 2.2, respectively). Microbial activity was higher in phenol-amended ground water as determined by measuring the reduction of 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium chloride. Thus phenol amendments to groundwater correlated with increased TCE mineralization, a decrease in diversity of the amplified microbial rDNA clone library, and increased microbial activity.Key words: community structure, trichloroethylene, degradation, groundwater.


Chemosphere ◽  
2016 ◽  
Vol 160 ◽  
pp. 1-6 ◽  
Author(s):  
Xiang Zhang ◽  
Xiaogang Gu ◽  
Shuguang Lu ◽  
Zhouwei Miao ◽  
Minhui Xu ◽  
...  

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