Flagella and Their Properties Affect the Transport and Deposition Behaviors of Escherichia coli in Quartz Sand

2021 ◽  
Author(s):  
Mengya Zhang ◽  
Lei He ◽  
Xin Jin ◽  
Fan Bai ◽  
Meiping Tong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quartz Sand

Flagella and their property affect the transport and deposition behaviors of Escherichia coli in quartz sand

2021 ◽  
Author(s):  
Mengya Zhang ◽  
Lei He ◽  
Meiping Tong
Keyword(s):  
Escherichia Coli ◽  
Porous Media ◽  
Quartz Sand ◽  
Packed Column ◽  
High Mobility ◽  
Deposition Process ◽  
Flow Chamber ◽  
Chamber System ◽  
Bacterial Flagella ◽  
Silica Surfaces

<p>The effects of bacterial flagella as well as their property on the transport and deposition of bacteria were examined by using four types of <em>Escherichia coli </em>(<em>E.coli</em>) with or without flagella, as well as with normal or sticky flagella. Packed column, quartz crystal microbalance with dissipation (QCM-D), visible parallel plate flow chamber system, as well as visible flow chamber system packed with porous media system were utilized to investigate the deposition behaviors and the deposition mechanisms of bacteria with different property of flagella. We found that the presence of flagella favored <em>E.coli</em> deposition onto quartz sand/silica surfaces. Moreover, by changing the porous media porosity and directly observing the deposition process of bacteria in porous media, grain-to-grain contacts were found to be major sites for bacterial deposition. Particularly, flagella could help bacteria swim near and then deposit at grain-to-grain contacts. In addition, we found that due to the stronger adhesive forces, sticky flagella could further enhance bacterial deposition onto quartz sand/silica surfaces. Elution experiments showed that the portion of bacteria with flagella depositing onto secondary energy minima was relatively lower than bacteria without flagella, indicating that flagella could help bacteria attach onto sand surfaces more irreversibly. Clearly, flagella and their property would have obvious influence on the transport and deposition behaviors of bacteria in porous media. By removing the flagella or changing their property, the transport and deposition of bacteria in porous media can be altered. Particularly, bacterial flagella can be removed to facilitate the transport of bacteria in remediation system requiring high mobility of bacteria, while in system requiring the immobilization bacteria in porous media, bacteria with sticky flagella can be employed.</p>

Towards understanding inter-strain attachment variations of Escherichia coli during transport in saturated quartz sand

2010 ◽  
Vol 44 (4) ◽  
pp. 1202-1212 ◽  
Author(s):  
Jan Willem Foppen ◽  
George Lutterodt ◽  
Wilfred F.M. Röling ◽  
Stefan Uhlenbrook
Keyword(s):  
Escherichia Coli ◽  
Quartz Sand

Transport of Escherichia coli in 25m quartz sand columns

2011 ◽  
Vol 119 (1-4) ◽  
pp. 80-88 ◽  
Author(s):  
G. Lutterodt ◽  
J.W.A. Foppen ◽  
A. Maksoud ◽  
S. Uhlenbrook
Keyword(s):  
Escherichia Coli ◽  
Quartz Sand

Effects of Retention in Effluent from a Wastewater Treatment Plant on Deposition of Escherichia coli in Packed-Bed Columns

2014 ◽  
Vol 955-959 ◽  
pp. 951-955
Author(s):  
Jiu Yi Li ◽  
Xiao Kang Zhao ◽  
Xiu Jun Tian ◽  
Jin Li ◽  
Li Jing Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Surface Properties ◽  
Wastewater Treatment Plant ◽  
Quartz Sand ◽  
Treatment Plant ◽  
Packed Bed ◽  
Bacterial Surface ◽  
The Impact ◽  
Packed Bed Columns

The transport of pathogenic bacteria from wastewater treatment facilities in subsurface has attracted extensive concerns over recent decades, while the impact of effluent water chemistry remains unclear. The influence of retention time in effluent from a municipal wastewater treatment plant on bacterial surface properties and deposition ofEscherichia colistrains in saturated quartz sand packed-bed columns was investigated in this paper. Retentions in effluent significantly altered bacterial surface properties, such as zeta potential, surface charge and hydrophobicity, subsequently changed their deposition rate coefficients and attachment efficiencies in quartz sand packed-bed columns. Under low ionic strength conditions, bacterial deposition onto quartz sand grains was in agreement with the predictions of DLVO theory, in which the secondary energy minimum was demonstrated to be predominant.

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

Sign in / Sign up

Export Citation Format

Share Document