Oxidative Stress, Endocrine Disturbance, and Immune Interference in Humans Showed Relationships to Serum Bisphenol Concentrations in a Dense Industrial Area

Chuanzi Gao; Haihong He; Wenhui Qiu; Yi Zheng; Yuyang Chen; Shiyao Hu; Xue Zhao

doi:10.1021/acs.est.0c07587

The Relationship Between Allergic Responses and Oxidative Stress Among Male Adults in an Industrial Area

Korean Journal of Environmental Health Sciences ◽

10.5668/jehs.2008.34.2.117 ◽

2008 ◽

Vol 34 (2) ◽

pp. 117-123 ◽

Cited By ~ 1

Author(s):

Young-Sun Ki ◽

Jin-Young Min ◽

Hong-Sik Yoo ◽

Do-Myung Paek

Keyword(s):

Oxidative Stress ◽

Industrial Area ◽

The Relationship ◽

And Oxidative Stress

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p-Nonylphenol Impairment of Osteogenic Differentiation of Mesenchymal Stem Cells was Found to be Due to Oxidative Stress and Down-Regulation of RUNX2 and BMP

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200505114058 ◽

2020 ◽

Vol 20 (8) ◽

pp. 1336-1346

Author(s):

Abnosi M. Hussein ◽

Masoomi Sina

Keyword(s):

Oxidative Stress ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Public Awareness ◽

Industrial Area ◽

Environmental Pollutant ◽

Electrolyte Imbalance ◽

Alizarin Red ◽

Total Antioxidant

Objectives: Previously, it was found that the para-nonylphenol (p-NP) impairs the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs); thus the aim of the present study was to evaluate the mechanism of the impairment. Methods: rBMSCs after 3rd passage cultured in osteogenic media in the presence of 0, 0.5 and 2.5 μM p-NP for 5, 10, 15 and 20 days. The study investigated the viability of the cells using MTT assays. The mineralization was studied using Alizarin red quantification analysis. Using a flame-photometer, the electrolytes (sodium and potassium) were measured, and the level of calcium as well as ALT, AST, ALP and LDH was determined by commercial kits. The level of total-antioxidant, MDA and the activity of SOD and CAT were estimated with the help of a spectrophotometer. Gene expression was studied using rt-PCR. Results: The p-NP treatment of osteogenic differentiated MSCs showed intracellular electrolyte imbalance and variation of cellular metabolism. In addition, we observed oxidative stress due to the reduction of total antioxidant capacity and the imbalance of antioxidant enzymes activity. Investigating the genes involved in the osteogenic differentiation of MSCs to osteoblast showed that the 2.5 μM of p-NP reduced the expression of the ALP, SMAD, BMP and RUNX2 genes. Conclusion: The study concludes that this pollutant via influencing the genomics and metabolic imbalance, as well as oxidative induction, caused a reduction of mineralization and differentiation of MSCs. This environmental pollutant might cause osteoporosis, which necessitates raising public awareness, especially to those who live in the industrial area to prevent its drastic effect.

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Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

Food & Function ◽

10.1039/d0fo01638a ◽

2020 ◽

Vol 11 (10) ◽

pp. 8547-8559

Author(s):

Hongjing Zhao ◽

Yu Wang ◽

Mengyao Mu ◽

Menghao Guo ◽

Hongxian Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Human Health ◽

Grass Carp ◽

Aquatic Environment ◽

Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Biochemical Journal ◽

10.1042/bcj20190591 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3705-3719 ◽

Cited By ~ 2

Author(s):

Avani Vyas ◽

Umamaheswar Duvvuri ◽

Kirill Kiselyov

Keyword(s):

Oxidative Stress ◽

Head And Neck ◽

Transcriptional Activation ◽

Platinum Resistance ◽

Mrna Levels ◽

Strong Positive Correlation ◽

Platinum Compounds ◽

Copper Chelation ◽

Novel Approach ◽

Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

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Clinical aspects of reactive oxygen and nitrogen species

Biochemical Society Symposium ◽

10.1042/bss0710121 ◽

2004 ◽

Vol 71 ◽

pp. 121-133 ◽

Cited By ~ 25

Author(s):

Ascan Warnholtz ◽

Maria Wendt ◽

Michael August ◽

Thomas Münzel

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Risk Factor ◽

Endothelial Dysfunction ◽

Vascular Tissue ◽

Rapid Development ◽

Reactive Oxygen ◽

Clinical Aspects ◽

No Production ◽

Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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