scholarly journals Potential Sensitivity of Wastewater Monitoring for SARS-CoV-2: Comparison with Norovirus Cases

2020 ◽  
Vol 54 (11) ◽  
pp. 6451-6452 ◽  
Author(s):  
Akihiko Hata ◽  
Ryo Honda
1987 ◽  
Vol 252 (1) ◽  
pp. C105-C114 ◽  
Author(s):  
T. G. Wingrove ◽  
G. A. Kimmich

Epithelial cells isolated from chick small intestine were used to study the mechanism of L-aspartate transport. Two kinetically distinct uptake systems of high (Km' = 16 microM) and low (Km'' = 2.7 mM) affinity are observed. This paper examines the cation dependence and membrane potential sensitivity of the high affinity system. Unidirectional influx studies indicate that extracellular Na+ is an absolute requirement for transport function. Flux is optimal when K+ is present intracellularly, however this cation is not required for Na+-dependent L-aspartate uptake. In the absence of K+, flux enhancement is observed when the intracellular pH is acidic. In contrast, acidic intracellular pH is inhibitory in cells that are preequilibrated with K+. Sodium ([Na+]o greater than [Na+]i gradients, and potassium ([K+]o less than [K+]i) or proton ([H+]o less than [H+]i) gradients can independently energize the Na+-dependent accumulation of L-aspartate above equilibrium levels, suggesting that Na+ and L-aspartate cotransport occurs with concomitant K+ or H+ antiport. L-Aspartate influx is insensitive to membrane potential changes created by inwardly directed anion gradients in the presence or absence of intracellular K+. A model is presented that is consistent with electroneutral Na+-coupled transfer with an ion antiport site of low specificity.


2012 ◽  
Vol 100 (22) ◽  
pp. 223104 ◽  
Author(s):  
Yasuhiro Sugawara ◽  
Lili Kou ◽  
Zongmin Ma ◽  
Takeshi Kamijo ◽  
Yoshitaka Naitoh ◽  
...  

1993 ◽  
Vol 87 (4) ◽  
pp. 221-234 ◽  
Author(s):  
A.P. Rudell ◽  
R.Q. Cracco ◽  
N.F. Hassan ◽  
L.P. Eberle

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