scholarly journals Role of Base Excision “Repair” Enzymes in Erasing Epigenetic Marks from DNA

2016 ◽  
Vol 116 (20) ◽  
pp. 12711-12729 ◽  
Author(s):  
Alexander C. Drohat ◽  
Christopher T. Coey
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Epigenetic Marks ◽  
Repair Enzymes ◽  
Base Excision

scholarly journals A distinct physiological role of MutY in mutation prevention in mycobacteria

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 88-93 ◽  
Author(s):  
Krishna Kurthkoti ◽  
Thiruneelakantan Srinath ◽  
Pradeep Kumar ◽  
Vidyasagar S. Malshetty ◽  
Pau Biak Sang ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Mycobacterium Smegmatis ◽  
Biochemical Characterization ◽  
Excision Repair ◽  
Physiological Role ◽  
Repair Enzymes ◽  
Base Excision ◽  
Oxidative Damage To Dna

Oxidative damage to DNA results in the occurrence of 7,8-dihydro-8-oxoguanine (8-oxoG) in the genome. In eubacteria, repair of such damage is initiated by two major base-excision repair enzymes, MutM and MutY. We generated a MutY-deficient strain of Mycobacterium smegmatis to investigate the role of this enzyme in DNA repair. The MutY deficiency in M. smegmatis did not result in either a noteworthy susceptibility to oxidative stress or an increase in the mutation rate. However, rifampicin-resistant isolates of the MutY-deficient strain showed distinct mutations in the rifampicin-resistance-determining region of rpoB. Besides the expected C to A (or G to T) mutations, an increase in A to C (or T to G) mutations was also observed. Biochemical characterization of mycobacterial MutY (M. smegmatis and M. tuberculosis) revealed an expected excision of A opposite 8-oxoG in DNA. Additionally, excision of G and T opposite 8-oxoG was detected. MutY formed complexes with DNA containing 8-oxoG : A, 8-oxoG : G or 8-oxoG : T but not 8-oxoG : C pairs. Primer extension reactions in cell-free extracts of M. smegmatis suggested error-prone incorporation of nucleotides into the DNA. Based on these observations, we discuss the physiological role of MutY in specific mutation prevention in mycobacteria.

scholarly journals Damage sensor role of UV-DDB during base excision repair

2019 ◽  
Vol 26 (8) ◽  
pp. 695-703 ◽  
Author(s):  
Sunbok Jang ◽  
Namrata Kumar ◽  
Emily C. Beckwitt ◽  
Muwen Kong ◽  
Elise Fouquerel ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Base Excision

scholarly journals The Role of Base Excision Repair in the Sensitivity and Resistance to Temozolomide-Mediated Cell Death

2005 ◽  
Vol 65 (14) ◽  
pp. 6394-6400 ◽  
Author(s):  
Ram N. Trivedi ◽  
Karen H. Almeida ◽  
Jamie L. Fornsaglio ◽  
Sandra Schamus ◽  
Robert W. Sobol
Keyword(s):  
Cell Death ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Base Excision
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