An Evolutionarily Conserved Subdomain in Amelotin Promotes Amorphous Calcium Phosphate-to-Hydroxyapatite Phase Transition

2019 ◽  
Vol 19 (4) ◽  
pp. 2104-2113 ◽  
Author(s):  
Menghan Yu ◽  
Lijun Wang ◽  
Wenjun Zhang ◽  
Bernhard Ganss
Keyword(s):  
Phase Transition ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Evolutionarily Conserved ◽  
Hydroxyapatite Phase

scholarly journals Reversal of Root Caries with Casein Phosphopeptide-Amorphous Calcium Phosphate and Fluoride Varnish in Xerostomia

2021 ◽  
pp. 1-10
Author(s):  
Ahmed Sleibi ◽  
Anwar R. Tappuni ◽  
Aylin Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Primary Root ◽  
Severity Index ◽  
Salivary Flow ◽  
Root Caries ◽  
Carious Lesions ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Group 1

Different formulas of topical fluoride have been used to manage root carious lesions. This clinical trial aimed to investigate the efficacy of a dental varnish containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride compared with fluoride alone in reversing/arresting root caries in xerostomic patients over 1 year. A total of 80 patients (age range 45–92 years) with primary root caries (<i>n</i> = 184 root carious lesions) and unstimulated salivary flow rate of &#x3c;0.2 mL/min were randomly allocated to receive either dental varnish containing CPP-ACP and 5% fluoride (group 1: MI varnish; GC, Japan) (<i>n</i> = 41, 83 lesions), or dental varnish with 5% fluoride alone (group 2: NUPRO White; Dentsply, USA) (<i>n</i> = 39, 101 lesions). Clinical assessments with Severity Index (SI) for root caries, DIAGNOdent measurements, and varnish application were carried out at baseline, 3, 6, and 12 months. Standard oral hygiene instructions with 1,450 ppm fluoride toothpastes were provided for both groups. After 3 months, 63.9% (<i>n</i> = 46) of root caries in group 1 became hard (SI: 0) compared with 39.3% (<i>n</i> = 35) in group 2 (<i>p</i> &#x3c; 0.01). After 6 and 12 months, the differences in SI were insignificant (group 1, <i>n</i> = 60, 83.3%) (group 2, <i>n</i> = 66, 74.2%) (<i>p</i> = 0.36), and (group 1, <i>n</i> = 60, 89.6%) (group 2, <i>n</i> = 67, 81.7%, <i>n</i> = 1 soft, 1.2%) (<i>p</i> = 0.29), respectively. In both groups, noncavitated leathery lesions were more likely to become hard when compared to the cavitated root caries. A significant decrease in plaque index, surface roughness, lesion dimension, and DIAGNOdent readings with a significant increase in lesion distance from the gingival margin was reported in both groups (<i>p</i> &#x3c; 0.05). This study has provided evidence that fluoride dental varnish either with or without calcium and phosphate has the potential to arrest/reverse root caries, especially noncavitated lesions for patients with xerostomia.

Impacts of Initial Ca/P on Amorphous Calcium Phosphate

2021 ◽  
Author(s):  
Alexandria J. Hoeher ◽  
Sebastian T. Mergelsberg ◽  
Olaf J. Borkiewicz ◽  
F. Marc Michel
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate

Casein phosphopeptide–amorphous calcium phosphate fluoride treatment enriches the symbiotic dental plaque microbiome in children

2021 ◽  
Vol 106 ◽  
pp. 103582
Author(s):  
Armelia Sari Widyarman ◽  
Nadeeka S. Udawatte ◽  
Citra Fragrantia Theodorea ◽  
Anie Apriani ◽  
Mario Richi ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Dental Plaque ◽  
Fluoride Treatment ◽  
Casein Phosphopeptide

scholarly journals Calcium phosphate phase transition in the presence of Aminosilane

2014 ◽  
Vol 70 (a1) ◽  
pp. C67-C67
Author(s):  
Babak Mostaghaci ◽  
Brigitta Loretz ◽  
Robert Haberkorn ◽  
Guido Kickelbick ◽  
Claus-Michael Lehr
Keyword(s):  
Phase Transition ◽  
Calcium Phosphate ◽  
Blood Compatibility ◽  
Transfection Efficiency ◽  
Hek293 Cells ◽  
Step Method ◽  
Calcium Phosphate Nanoparticles ◽  
Amine Groups ◽  
The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

scholarly journals A safer disposal of hazardous phosphate coating sludge by formation of an amorphous calcium phosphate matrix

2015 ◽  
Vol 159 ◽  
pp. 288-300 ◽  
Author(s):  
I. Navarro-Blasco ◽  
A. Duran ◽  
M. Pérez-Nicolás ◽  
J.M. Fernández ◽  
R. Sirera ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Phosphate Coating

Enzymatic Synthesis of Amorphous Calcium Phosphate−Chitosan Nanocomposites and Their Processing into Hierarchical Structures

2008 ◽  
Vol 20 (1) ◽  
pp. 11-13 ◽  
Author(s):  
María C. Gutiérrez ◽  
Matías Jobbágy ◽  
María L. Ferrer ◽  
Francisco del Monte
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Enzymatic Synthesis ◽  
Hierarchical Structures
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