Directed Assembly of acac-Based Complexes by Deliberately Fine-Tuning Electrostatic Molecular-Recognition Events

2016 ◽  
Vol 16 (12) ◽  
pp. 7308-7317 ◽  
Author(s):  
Boris-Marko Kukovec ◽  
Magdalena Malik ◽  
Ivan Kodrin ◽  
Christer B. Aakeröy ◽  
Marijana Đaković
Keyword(s):  
Molecular Recognition ◽  
Directed Assembly ◽  
Fine Tuning

Supramolecular DNA nanotechnology

2009 ◽  
Vol 81 (12) ◽  
pp. 2157-2181 ◽  
Author(s):  
Faisal A. Aldaye ◽  
Hanadi F. Sleiman
Keyword(s):  
Molecular Recognition ◽  
Base Pair ◽  
Deoxyribonucleic Acid ◽  
Materials Science ◽  
Dna Nanotechnology ◽  
Structure And Function ◽  
Fine Tuning ◽  
Functional Potential ◽  
And Function ◽  
Main Material

Nature uses deoxyribonucleic acid (DNA) as the main material for the storage and transmission of life’s blueprint. Today, DNA is being used as a “smart” material to help solve a number of long-standing issues facing researchers in materials science and nanotechnology. In DNA nanotechnology, DNA’s powerful base-pair molecular recognition criteria are utilized to control the final structure and function of the material being generated. A sub-area of research that our group has recently termed “supramolecular DNA nanotechnology” is emerging and is extending the limits of this molecule in nanotechnology by further fine-tuning DNA’s structural and functional potential. This review will discuss the fruition and fundamentals of supramolecular DNA nanotechnology, as well as its future as a viable science in a material world.

scholarly journals Assembly/Disassembly of DNA-Au Nanoparticles: A Strategy of Intervention

2008 ◽  
Vol 2008 ◽  
pp. 1-4 ◽  
Author(s):  
I-Im S. Lim ◽  
Lingyan Wang ◽  
Uma Chandrachud ◽  
Susannah Gal ◽  
Chuan-Jian Zhong
Keyword(s):  
Gold Nanoparticles ◽  
Optical Properties ◽  
Molecular Recognition ◽  
Au Nanoparticles ◽  
Model System ◽  
Fine Tuning ◽  
Preliminary Results ◽  
Recognition Probe

This report describes the viability of a strategy for manipulating the assembly/disassembly processes of DNA-Au nanoparticles by molecular intervention. Using the temperature-induced assembly and disassembly processes of DNAs and gold nanoparticles as a model system, the introduction of a molecular recognition probe is demonstrated to lead to the intervention of the assembly/disassembly processes depending on its specific biorecognition. This process can be detected by monitoring the change in the optical properties of gold nanoparticles and their DNA assemblies. Implications of the preliminary results to exploration of the resulting nanostructures for fine-tuning of the interfacial reactivities in DNA-based bioassays and biomaterial engineering are also discussed.

Fine-Tuning Ligand−Receptor Design for Selective Molecular Recognition of Dicarboxylic Acids

2007 ◽  
Vol 46 (25) ◽  
pp. 10632-10638 ◽  
Author(s):  
Arnau Arbuse ◽  
Carmen Anda ◽  
Ma Angeles Martínez ◽  
Javier Pérez-Mirón ◽  
Carlos Jaime ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Dicarboxylic Acids ◽  
Fine Tuning ◽  
Receptor Design

scholarly journals Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

2018 ◽  
Vol 115 (31) ◽  
pp. E7293-E7302 ◽  
Author(s):  
Charlotte M. Miton ◽  
Stefanie Jonas ◽  
Gerhard Fischer ◽  
Fernanda Duarte ◽  
Mark F. Mohamed ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Transition State ◽  
Active Site ◽  
High Reactivity ◽  
Fine Tuning ◽  
Binding Modes ◽  
Evolutionary Trajectory ◽  
Multiple Substrates ◽  
Enzyme Substrate ◽  
Multiple Substrate

The recruitment and evolutionary optimization of promiscuous enzymes is key to the rapid adaptation of organisms to changing environments. Our understanding of the precise mechanisms underlying enzyme repurposing is, however, limited: What are the active-site features that enable the molecular recognition of multiple substrates with contrasting catalytic requirements? To gain insights into the molecular determinants of adaptation in promiscuous enzymes, we performed the laboratory evolution of an arylsulfatase to improve its initially weak phenylphosphonate hydrolase activity. The evolutionary trajectory led to a 100,000-fold enhancement of phenylphosphonate hydrolysis, while the native sulfate and promiscuous phosphate mono- and diester hydrolyses were only marginally affected (≤50-fold). Structural, kinetic, and in silico characterizations of the evolutionary intermediates revealed that two key mutations, T50A and M72V, locally reshaped the active site, improving access to the catalytic machinery for the phosphonate. Measured transition state (TS) charge changes along the trajectory suggest the creation of a new Michaelis complex (E•S, enzyme–substrate), with enhanced leaving group stabilization in the TS for the promiscuous phosphonate (βleavinggroup from −1.08 to −0.42). Rather than altering the catalytic machinery, evolutionary repurposing was achieved by fine-tuning the molecular recognition of the phosphonate in the Michaelis complex, and by extension, also in the TS. This molecular scenario constitutes a mechanistic alternative to adaptation solely based on enzyme flexibility and conformational selection. Instead, rapid functional transitions between distinct chemical reactions rely on the high reactivity of permissive active-site architectures that allow multiple substrate binding modes.

Fine-Tuning Futures

ASHA Leader ◽  
2017 ◽  
Vol 22 (6) ◽  
Author(s):  
Christi Miller
Keyword(s):  
Fine Tuning

Food Safety Security: A new Concept for Enhancing Food Safety Measures

2012 ◽  
Vol 82 (3) ◽  
pp. 216-222 ◽  
Author(s):  
Venkatesh Iyengar ◽  
Ibrahim Elmadfa
Keyword(s):  
Food Safety ◽  
Hazard Analysis ◽  
Warning System ◽  
Shared Responsibility ◽  
Fine Tuning ◽  
Strategic Action ◽  
Surveillance Network ◽  
Measurement Systems ◽  
Critical Control Points ◽  
The Impact

The food safety security (FSS) concept is perceived as an early warning system for minimizing food safety (FS) breaches, and it functions in conjunction with existing FS measures. Essentially, the function of FS and FSS measures can be visualized in two parts: (i) the FS preventive measures as actions taken at the stem level, and (ii) the FSS interventions as actions taken at the root level, to enhance the impact of the implemented safety steps. In practice, along with FS, FSS also draws its support from (i) legislative directives and regulatory measures for enforcing verifiable, timely, and effective compliance; (ii) measurement systems in place for sustained quality assurance; and (iii) shared responsibility to ensure cohesion among all the stakeholders namely, policy makers, regulators, food producers, processors and distributors, and consumers. However, the functional framework of FSS differs from that of FS by way of: (i) retooling the vulnerable segments of the preventive features of existing FS measures; (ii) fine-tuning response systems to efficiently preempt the FS breaches; (iii) building a long-term nutrient and toxicant surveillance network based on validated measurement systems functioning in real time; (iv) focusing on crisp, clear, and correct communication that resonates among all the stakeholders; and (v) developing inter-disciplinary human resources to meet ever-increasing FS challenges. Important determinants of FSS include: (i) strengthening international dialogue for refining regulatory reforms and addressing emerging risks; (ii) developing innovative and strategic action points for intervention {in addition to Hazard Analysis and Critical Control Points (HACCP) procedures]; and (iii) introducing additional science-based tools such as metrology-based measurement systems.

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