Protein Crystallization from a Preordered Metastable Intermediate Phase Followed by Real-Time Small-Angle Neutron Scattering

2021 ◽  
Author(s):  
Ralph Maier ◽  
Benedikt Sohmen ◽  
Stefano Da Vela ◽  
Olga Matsarskaia ◽  
Christian Beck ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Real Time ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Intermediate Phase ◽  
Protein Crystallization

scholarly journals Vortex fluidic mediated encapsulation of functional fish oil featuring in situ probed small angle neutron scattering

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Shan He ◽  
Nikita Joseph ◽  
Marzieh Mirzamani ◽  
Scott J. Pye ◽  
Ahmed Hussein Mohammed Al-anataki ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Neutron Scattering ◽  
Real Time ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Bioactive Molecules ◽  
Omega 3 Fatty Acids ◽  
Omega 3

Abstract Major challenges for optimizing the benefits of fish oil on human health are improved bioavailability while overcoming the strong odor and avoiding significant oxidation of the omega-3 polyunsaturated fatty acids (PUFAs). The scalable continuous flow thin film vortex fluidic device (VFD) improves the Tween 20 encapsulation of fish oil relative to conventional homogenization processing, with the fish oil particles significantly smaller and the content of the valuable omega-3 fatty acids higher. In addition, after 14 days storage the remaining omega-3 fatty acids content was higher, from ca 31.0% for raw fish oil to ca 62.0% of freeze-dried encapsulated fish oil. The VFD mediated encapsulated fish oil was used to enrich the omega-3 fatty acid content of apple juice, as a model water-based food product, without changing its sensory values. The versatility of the VFD was further demonstrated in forming homogenous suspensions of fish oil containing water-insoluble bioactive molecules, curcumin and quercetin. We have also captured, for the first time, real-time structural changes in nanoencapsulation by installing a VFD with in in situ small angle neutron scattering. Real-time measurements afford valuable insights about self-assembly in solution.

Probing the dynamic self-assembly behaviour of photoswitchable wormlike micelles in real-time

Soft Matter ◽  
2019 ◽  
Vol 15 (6) ◽  
pp. 1253-1259 ◽  
Author(s):  
Elaine A. Kelly ◽  
Judith E. Houston ◽  
Rachel C. Evans
Keyword(s):  
Neutron Scattering ◽  
Real Time ◽  
Absorption Spectroscopy ◽  
Small Angle ◽  
Self Assembly ◽  
Small Angle Neutron Scattering ◽  
Wormlike Micelles

UV-Vis absorption spectroscopy is combined with small-angle neutron scattering to monitor the dynamic self-assembly of an azobenzene photosurfactant from worm-like micelles to fractals during photoisomerization.

scholarly journals Elongation rate and average length of amyloid fibrils in solution using isotope-labelled small-angle neutron scattering

2021 ◽  
Author(s):  
Ben Jonathon Eves ◽  
James Doutch ◽  
Ann E Terry ◽  
Han Yin ◽  
Martine Moulin ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Real Time ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Solution Method ◽  
Amyloid Fibrils ◽  
Average Length ◽  
Elongation Rate ◽  
Scattering Data ◽  
Fibril Length

We demonstrate a solution method that allows both elongation rate and average fibril length of assembling amyloid fibrils to be estimated. The approach involves acquisition of real-time neutron scattering data...

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