Rapid Analysis for Multicomponent High-Throughput Crystallization Screening: Combination of UV–Vis Spectroscopy and Chemometrics

2022 ◽  
Author(s):  
Christina H. Wegner ◽  
Ines Zimmermann ◽  
Jürgen Hubbuch
Keyword(s):  
High Throughput ◽  
Rapid Analysis ◽  
Vis Spectroscopy

scholarly journals Clinical Analysis by Microchip Capillary Electrophoresis

2006 ◽  
Vol 52 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Sam FY Li ◽  
Larry J Kricka
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
Early Stage ◽  
Clinical Analysis ◽  
Rapid Analysis ◽  
Clinical Testing ◽  
Parallel Operation ◽  
Microchip Capillary Electrophoresis ◽  
Stage Of Development ◽  
Easy Integration

Abstract Clinical analysis often requires rapid, automated, and high-throughput analytical systems. Microchip capillary electrophoresis (CE) has the potential to achieve very rapid analysis (typically seconds), easy integration of multiple analytical steps, and parallel operation. Although it is currently still in an early stage of development, there are already many reports in the literature describing the applications of microchip CE in clinical analysis. At the same time, more fully automated and higher throughput commercial instruments for microchip CE are becoming available and are expected to further enhance the development of applications of microchip CE in routine clinical testing. To put into perspective its potential, we briefly compare microchip CE with conventional CE and review developments in this technique that may be useful in diagnosis of major diseases.

A High-Throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates

ACS Nano ◽  
2019 ◽  
Vol 13 (10) ◽  
pp. 11955-11966 ◽  
Author(s):  
Simon Sehayek ◽  
Yasser Gidi ◽  
Viktorija Glembockyte ◽  
Hugo B. Brandão ◽  
Paul François ◽  
...  
Keyword(s):  
High Throughput ◽  
Correlation Method ◽  
Rapid Analysis ◽  
Image Correlation

scholarly journals Monitoring Hydroxycinnamic Acid Decarboxylation by Lactic Acid Bacteria Using High-Throughput UV-Vis Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3142 ◽  
Author(s):  
Gonzalo Miyagusuku-Cruzado ◽  
Israel García-Cano ◽  
Diana Rocha-Mendoza ◽  
Rafael Jiménez-Flores ◽  
M. Monica Giusti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Caffeic Acid ◽  
High Throughput ◽  
Hydroxycinnamic Acid ◽  
Acid Decarboxylase ◽  
State University ◽  
Coumaric Acid ◽  
Vis Spectroscopy ◽  
The Ohio State University

Hydroxycinnamic acid (HCA) decarboxylation by lactic acid bacteria (LAB) results in the production of 4-vinylplenols with great impact on the sensorial characteristics of foods. The determination of LAB decarboxylating capabilities is key for optimal strain selection for food production. The activity of LAB strains from the Ohio State University—Parker Endowed Chair (OSU-PECh) collection potentially capable of synthesizing phenolic acid decarboxylase was evaluated after incubation with HCAs for 36 h at 32 °C. A high-throughput method for monitoring HCAs decarboxylation was developed based on hypsochromic shifts at pH 1.0. Out of 22 strains evaluated, only Enterococcus mundtii, Lactobacillus plantarum and Pediococcus pentosaceus were capable of decarboxylating all p-coumaric, caffeic and ferulic acids. Other strains only decarboxylated p-coumaric and caffeic acid (6), only p-coumaric acid (2) or only caffeic acid (1), while 10 strains did not decarboxylate any HCA. p-Coumaric acid had the highest conversion efficiency, followed by caffeic acid and lastly ferulic acid. Results were confirmed by HPLC-DAD-ESI-MS analyses, showing the conversion of HCAs into their 4-vinylphenol derivatives. This work can help improve the sensory characteristics of HCA-rich foods where fermentation with LAB was used during processing.

scholarly journals A combined high-throughput and high-content platform for unified on-chip synthesis, characterization and biological screening

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Maximilian Benz ◽  
Arndt Asperger ◽  
Meike Hamester ◽  
Alexander Welle ◽  
Stefan Heissler ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
Indium Tin Oxide ◽  
Oxide Coating ◽  
Ionization Mass ◽  
Drug Discovery Process ◽  
Biological Screening ◽  
Vis Spectroscopy ◽  
On Chip

Abstract Acceleration and unification of drug discovery is important to reduce the effort and cost of new drug development. Diverse chemical and biological conditions, specialized infrastructure and incompatibility between existing analytical methods with high-throughput, nanoliter scale chemistry make the whole drug discovery process lengthy and expensive. Here, we demonstrate a chemBIOS platform combining on-chip chemical synthesis, characterization and biological screening. We developed a dendrimer-based surface patterning that enables the generation of high-density nanodroplet arrays for both organic and aqueous liquids. Each droplet (among > 50,000 droplets per plate) functions as an individual, spatially separated nanovessel, that can be used for solution-based synthesis or analytical assays. An additional indium-tin oxide coating enables ultra-fast on-chip detection down to the attomole per droplet by matrix-assisted laser desorption/ionization mass spectrometry. The excellent optical properties of the chemBIOS platform allow for on-chip characterization and in-situ reaction monitoring in the ultraviolet, visible (on-chip UV-Vis spectroscopy and optical microscopy) and infrared (on-chip IR spectroscopy) regions. The platform is compatible with various cell-biological screenings, which opens new avenues in the fields of high-throughput synthesis and drug discovery.

scholarly journals A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

2019 ◽  
Vol 2 (3) ◽  
pp. 60 ◽  
Author(s):  
Felix Kaspar ◽  
Robert T. Giessmann ◽  
Niels Krausch ◽  
Peter Neubauer ◽  
Anke Wagner ◽  
...  
Keyword(s):  
High Throughput ◽  
Absolute Intensity ◽  
Spectral Unmixing ◽  
Experimental Procedure ◽  
Vis Spectroscopy ◽  
Sample Transfer ◽  
Plate Measurement ◽  
Speed Of Analysis ◽  
Hplc Analyses

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet modern requirements regarding cost, speed of analysis and high throughput. Herein, we present a UV/Vis spectroscopy-based assay employing an algorithm for spectral unmixing in a 96-well plate format. The algorithm relies on fitting of reference spectra of nucleosides and their bases to experimental spectra and allows determination of nucleoside/nucleobase ratios in solution with high precision. The experimental procedure includes appropriate dilution of a sample into aqueous alkaline solution, transfer to a multi-well plate, measurement of a UV/Vis spectrum and subsequent in silico spectral unmixing. This enables data collection in a high-throughput fashion and reduces costs compared to state-of-the-art HPLC analyses by approximately 5-fold while being 20-fold faster and offering comparable precision. Additionally, the method is robust regarding dilution and sample transfer errors as it only considers spectral form and not absolute intensity. It can be applied to all natural nucleosides and nucleobases and even unnatural ones as demonstrated by several examples.

scholarly journals A High-throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates

2020 ◽  
Vol 118 (3) ◽  
pp. 310a
Author(s):  
Simon Sehayek ◽  
Yasser Gidi ◽  
Viktorija Glembockyte ◽  
Hugo B. Brandao ◽  
Paul Francois ◽  
...  
Keyword(s):  
High Throughput ◽  
Correlation Method ◽  
Rapid Analysis ◽  
Image Correlation
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