scholarly journals Distinguishing Specific and Nonspecific Complexes of Alkyladenine DNA Glycosylase

Biochemistry ◽  
2018 ◽  
Vol 57 (30) ◽  
pp. 4440-4454 ◽  
Author(s):  
Erin L. Taylor ◽  
Preethi M. Kesavan ◽  
Abigail E. Wolfe ◽  
Patrick J. O’Brien
Keyword(s):  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

scholarly journals Isolating Contributions from Intersegmental Transfer to DNA Searching by Alkyladenine DNA Glycosylase

2013 ◽  
Vol 288 (34) ◽  
pp. 24550-24559 ◽  
Author(s):  
Mark Hedglin ◽  
Yaru Zhang ◽  
Patrick J. O'Brien
Keyword(s):  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

Target-mediated hyperbranched amplification for sensitive detection of human alkyladenine DNA glycosylase from HeLa cells

Talanta ◽  
2019 ◽  
Vol 194 ◽  
pp. 846-851 ◽  
Author(s):  
Lili Wang ◽  
Huige Zhang ◽  
Yi Xie ◽  
Hongli Chen ◽  
Cuiling Ren ◽  
...  
Keyword(s):  
Hela Cells ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

scholarly journals Human Alkyladenine DNA Glycosylase Employs a Processive Search for DNA Damage†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11434-11445 ◽  
Author(s):  
Mark Hedglin ◽  
Patrick J. O’Brien
Keyword(s):  
Dna Damage ◽  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

scholarly journals Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicola P. Montaldo ◽  
Diana L. Bordin ◽  
Alessandro Brambilla ◽  
Marcel Rösinger ◽  
Sarah L. Fordyce Martin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genome Stability ◽  
Genome Instability ◽  
Excision Repair ◽  
Regulation Of Gene Expression ◽  
Direct Interaction ◽  
Dna Glycosylase ◽  
Pol Ii ◽  
Naked Dna ◽  
Alkyladenine Dna Glycosylase

AbstractBase excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases, including cancer and neurological disorders. While BER is well studied on naked DNA, it remains unclear how BER efficiently operates on chromatin. Here, we show that AAG binds to chromatin and forms complex with RNA polymerase (pol) II. This occurs through direct interaction with Elongator and results in transcriptional co-regulation. Importantly, at co-regulated genes, aberrantly methylated bases accumulate towards the 3′end in regions enriched for BER enzymes AAG and APE1, Elongator and active RNA pol II. Active transcription and functional Elongator are further crucial to ensure efficient BER, by promoting AAG and APE1 chromatin recruitment. Our findings provide insights into genome stability maintenance in actively transcribing chromatin and reveal roles of aberrantly methylated bases in regulation of gene expression.

scholarly journals Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level

2018 ◽  
Vol 9 (3) ◽  
pp. 712-720 ◽  
Author(s):  
Juan Hu ◽  
Ming-hao Liu ◽  
Ying Li ◽  
Bo Tang ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Single Molecule ◽  
Simultaneous Detection ◽  
Sensitive Detection ◽  
Lung Cancer Cells ◽  
Dna Glycosylase ◽  
Dna Glycosylases ◽  
Single Molecule Level ◽  
Alkyladenine Dna Glycosylase

We demonstrate the simultaneous detection of human 8-oxoguanine DNA glycosylase 1 and human alkyladenine DNA glycosylase at the single-molecule level.

Repair of 1, N 2 ‐ethenoguanine by Alkyladenine DNA Glycosylase

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Adam Thelen ◽  
Patrick O’Brien
Keyword(s):  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

scholarly journals “Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression”

2019 ◽  
Author(s):  
Nicola P. Montaldo ◽  
Diana L. Bordin ◽  
Alessandro Brambilla ◽  
Marcel Rösinger ◽  
Sarah L. Fordyce Martin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genome Stability ◽  
Genome Instability ◽  
Excision Repair ◽  
Regulation Of Gene Expression ◽  
Direct Interaction ◽  
Dna Glycosylase ◽  
Pol Ii ◽  
Naked Dna ◽  
Alkyladenine Dna Glycosylase

AbstractBase excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG; aka MPG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases including cancer and neurological disorders. While BER is well studied on naked DNA, it remains unclear how BER efficiently operates on chromatin. Here we show that AAG binds to chromatin and forms complex with active RNA polymerase (pol) II. This occurs through direct interaction with Elongator and results in transcriptional co-regulation. Importantly, at co-regulated genes aberrantly methylated bases accumulate towards 3’end, in regions enriched for BER enzymes AAG and APE1, Elongator and active RNA pol II. Active transcription and functional Elongator are further crucial to ensure efficient BER, by promoting AAG and APE1 chromatin recruitment. Our findings provide novel insights to maintaining genome stability in actively transcribing chromatin, and reveal roles of aberrantly methylated bases in regulation of gene expression.

scholarly journals Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase

Biochemistry ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 382-390 ◽  
Author(s):  
Jeremy W. Setser ◽  
Gondichatnahalli M. Lingaraju ◽  
C. Ainsley Davis ◽  
Leona D. Samson ◽  
Catherine L. Drennan
Keyword(s):  
Dna Binding ◽  
Dna Lesions ◽  
Dna Glycosylase ◽  
Alkyladenine Dna Glycosylase

scholarly journals Substrate binding pocket residues of human alkyladenine-DNA glycosylase critical for methylating agent survival

DNA Repair ◽  
2008 ◽  
Vol 7 (10) ◽  
pp. 1731-1745 ◽  
Author(s):  
C CHEN ◽  
H GUO ◽  
D SHAH ◽  
A BLANK ◽  
L SAMSON ◽  
...  
Keyword(s):  
Substrate Binding ◽  
Binding Pocket ◽  
Dna Glycosylase ◽  
Substrate Binding Pocket ◽  
Alkyladenine Dna Glycosylase
Sign in / Sign up

Export Citation Format

Share Document