scholarly journals Exploration of the Effects of γ-Phosphate-Modified ATP Analogues on Histidine Kinase Autophosphorylation

Biochemistry ◽  
2018 ◽  
Vol 57 (29) ◽  
pp. 4368-4373 ◽  
Author(s):  
Olivia M. Chase ◽  
Adeline Espinasse ◽  
Kaelyn E. Wilke ◽  
Erin E. Carlson
Keyword(s):  
Histidine Kinase ◽  
Atp Analogues

scholarly journals Mechanistic Studies of Bioorthogonal ATP Analogues for Assessment of Histidine Kinase Autophosphorylation

2020 ◽  
Vol 15 (5) ◽  
pp. 1252-1260
Author(s):  
Adeline Espinasse ◽  
Xuelan Wen ◽  
Jason D. Goodpaster ◽  
Erin E. Carlson
Keyword(s):  
Histidine Kinase ◽  
Mechanistic Studies ◽  
Atp Analogues

scholarly journals Interhelical H-Bonds Modulate the Activity of a Polytopic Transmembrane Kinase

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 938
Author(s):  
Juan Cruz Almada ◽  
Ana Bortolotti ◽  
Jean Marie Ruysschaert ◽  
Diego de Mendoza ◽  
María Eugenia Inda ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Adaptive Response ◽  
Catalytic Domain ◽  
Membrane Lipids ◽  
Signal Transmission ◽  
Expression System ◽  
Lipid Homeostasis ◽  
Transmembrane Helices ◽  
Transmembrane Region

DesK is a Histidine Kinase that allows Bacillus subtilis to maintain lipid homeostasis in response to changes in the environment. It is located in the membrane, and has five transmembrane helices and a cytoplasmic catalytic domain. The transmembrane region triggers the phosphorylation of the catalytic domain as soon as the membrane lipids rigidify. In this research, we study how transmembrane inter-helical interactions contribute to signal transmission; we designed a co-expression system that allows studying in vivo interactions between transmembrane helices. By Alanine-replacements, we identified a group of polar uncharged residues, whose side chains contain hydrogen-bond donors or acceptors, which are required for the interaction with other DesK transmembrane helices; a particular array of H-bond- residues plays a key role in signaling, transmitting information detected at the membrane level into the cell to finally trigger an adaptive response.

scholarly journals Inhibition of vascular smooth muscle cell calcification by ATP analogues

2019 ◽  
Vol 15 (3) ◽  
pp. 315-326 ◽  
Author(s):  
Jessal J. Patel ◽  
Lucie E. Bourne ◽  
José Luis Millán ◽  
Timothy R. Arnett ◽  
Vicky E. MacRae ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Atp Analogues
Sign in / Sign up

Export Citation Format

Share Document