Kinetic Evidence for a Second Ligand Binding Site onStreptococcus pneumoniaePenicillin-Binding Protein 2x

Biochemistry ◽  
2018 ◽  
Vol 57 (11) ◽  
pp. 1758-1766
Author(s):  
S. A. Adediran ◽  
Kumar Subarno Sarkar ◽  
R. F. Pratt
Keyword(s):  
Ligand Binding ◽  
Binding Site ◽  
Binding Protein ◽  
Ligand Binding Site

scholarly journals Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

2011 ◽  
Vol 11 (1) ◽  
pp. 64 ◽  
Author(s):  
Tiina A Riihimäki ◽  
Soili Hiltunen ◽  
Martina Rangl ◽  
Henri R Nordlund ◽  
Juha AE Määttä ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Site ◽  
Binding Protein ◽  
Ligand Binding Site ◽  
Steroid Binding ◽  
Steroid Binding Protein

Structural analysis of the choline-binding protein ChoX in a semi-closed and ligand-free conformation

2009 ◽  
Vol 390 (11) ◽  
Author(s):  
Christine Oswald ◽  
Sander H.J. Smits ◽  
Marina Höing ◽  
Erhard Bremer ◽  
Lutz Schmitt
Keyword(s):  
Crystal Structure ◽  
Structural Analysis ◽  
Ligand Binding ◽  
Closed Form ◽  
Binding Site ◽  
Sinorhizobium Meliloti ◽  
Binding Protein ◽  
Ligand Binding Site ◽  
Abc Transport ◽  
Ligand Free

Abstract The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed but substrate-free state of ChoX. This latter structure revealed an architecture of the ligand-binding site that is superimposable to the closed, ligand-bound form of ChoX. We report here the crystal structure of ChoX in an unusual, ligand-free conformation that represents a semi-closed form of ChoX. The analysis revealed a subdomain movement in the N-lobe of ChoX. Comparison with the two well-characterized substrate binding proteins, MBP and HisJ, suggests the presence of a similar subdomain in these proteins.

scholarly journals Cysteine Mutagenesis and Homology Modeling of the Ligand-binding Site of a Kainate-binding Protein

1999 ◽  
Vol 274 (52) ◽  
pp. 37210-37218 ◽  
Author(s):  
Z. Galen Wo ◽  
Kamaldeep K. Chohan ◽  
Haiying Chen ◽  
Michael J. Sutcliffe ◽  
Robert E. Oswald
Keyword(s):  
Ligand Binding ◽  
Homology Modeling ◽  
Binding Site ◽  
Binding Protein ◽  
Ligand Binding Site ◽  
Cysteine Mutagenesis

Constraints of Opsin Structure on the Ligand-binding Site: Studies with Ring-fused Retinals¶

2002 ◽  
Vol 76 (6) ◽  
pp. 606 ◽  
Author(s):  
Takahiro Hirano ◽  
In Taek Lim ◽  
Don Moon Kim ◽  
Xiang-Guo Zheng ◽  
Kazuo Yoshihara ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Site ◽  
Ligand Binding Site
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