scholarly journals Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions

Biochemistry ◽  
2018 ◽  
Vol 57 (5) ◽  
pp. 540-546 ◽  
Author(s):  
Leonard Breindel ◽  
Christopher DeMott ◽  
David S. Burz ◽  
Alexander Shekhtman
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Real Time ◽  
Protein Interactions ◽  
Nuclear Magnetic

Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-13C]Fumarate

2019 ◽  
Vol 141 (51) ◽  
pp. 20209-20214 ◽  
Author(s):  
James Eills ◽  
Eleonora Cavallari ◽  
Carla Carrera ◽  
Dmitry Budker ◽  
Silvio Aime ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Real Time ◽  
Resonance Detection ◽  
Fumarase Activity ◽  
Nuclear Magnetic

scholarly journals A Nuclear Magnetic Resonance (NMR) Platform for Real-Time Metabolic Monitoring of Bioprocesses

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4675
Author(s):  
Ninad Mehendale ◽  
Felix Jenne ◽  
Chandrakant Joshi ◽  
Swati Sharma ◽  
Shyam Kumar Masakapalli ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Real Time ◽  
Orange Peel ◽  
High Spectral Resolution ◽  
Real Time Monitoring ◽  
Biochemical Reactions ◽  
Measurement Capability ◽  
User Intervention ◽  
Nuclear Magnetic

We present a Nuclear Magnetic Resonance (NMR) compatible platform for the automated real-time monitoring of biochemical reactions using a flow shuttling configuration. This platform requires a working sample volume of ∼11 mL and it can circulate samples with a flow rate of 28 mL/min, which makes it suitable to be used for real-time monitoring of biochemical reactions. Another advantage of the proposed low-cost platform is the high spectral resolution. As a proof of concept, we acquire 1H NMR spectra of waste orange peel, bioprocessed using Trichoderma reesei fungus, and demonstrate the real-time measurement capability of the platform. The measurement is performed over more than 60 h, with a spectrum acquired every 7 min, such that over 510 data points are collected without user intervention. The designed system offers high resolution, automation, low user intervention, and, therefore, time-efficient measurement per sample.

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