Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide

Biochemistry ◽  
2017 ◽  
Vol 56 (5) ◽  
pp. 779-792
Author(s):  
Valquíria Campos Alencar ◽  
Daniela Leite Jabes ◽  
Fabiano Bezerra Menegidio ◽  
Guilherme Lanzi Sassaki ◽  
Lucas Rodrigo de Souza ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Xylella Fastidiosa ◽  
Bacterial Lipopolysaccharide

scholarly journals Detection and characterization of protease secreted by the plant pathogen Xylella fastidiosa

2006 ◽  
Vol 161 (3) ◽  
pp. 263-272 ◽  
Author(s):  
Luciana Maria Fedatto ◽  
Maria Estela Silva-Stenico ◽  
Augusto Etchegaray ◽  
Flávia T.H. Pacheco ◽  
Jorge L.M. Rodrigues ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Xylella Fastidiosa

scholarly journals Novel Virulent Bacteriophages Infecting Mediterranean Isolates of the Plant Pest Xylella fastidiosa and Xanthomonas albilineans

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 725
Author(s):  
Fernando Clavijo-Coppens ◽  
Nicolas Ginet ◽  
Sophie Cesbron ◽  
Martial Briand ◽  
Marie-Agnès Jacques ◽  
...  
Keyword(s):  
Phage Therapy ◽  
Xylella Fastidiosa ◽  
Infected Plant ◽  
Mediterranean Area ◽  
Genomic Features ◽  
Phylogeny Analysis ◽  
Plant Pest ◽  
European Regulations ◽  
Surrogate Host

Xylella fastidiosa (Xf) is a plant pathogen causing significant losses in agriculture worldwide. Originating from America, this bacterium caused recent epidemics in southern Europe and is thus considered an emerging pathogen. As the European regulations do not authorize antibiotic treatment in plants, alternative treatments are urgently needed to control the spread of the pathogen and eventually to cure infected crops. One such alternative is the use of phage therapy, developed more than 100 years ago to cure human dysentery and nowadays adapted to agriculture. The first step towards phage therapy is the isolation of the appropriate bacteriophages. With this goal, we searched for phages able to infect Xf strains that are endemic in the Mediterranean area. However, as Xf is truly a fastidious organism, we chose the phylogenetically closest and relatively fast-growing organism X. albineans as a surrogate host for the isolation step. Our results showed the isolation from various sources and preliminary characterization of several phages active on different Xf strains, namely, from the fastidiosa (Xff), multiplex (Xfm), and pauca (Xfp) subspecies, as well as on X. albilineans. We sequenced their genomes, described their genomic features, and provided a phylogeny analysis that allowed us to propose new taxonomic elements. Among the 14 genomes sequenced, we could identify two new phage species, belonging to two new genera of the Caudoviricetes order, namely, Usmevirus (Podoviridae family) and Subavirus (Siphoviridae family). Interestingly, no specific phages could be isolated from infected plant samples, whereas one was isolated from vector insects captured in a contaminated area, and several from surface and sewage waters from the Marseille area.

scholarly journals Characterization of an ATP Translocase Identified in the Destructive Plant Pathogen “Candidatus Liberibacter asiaticus”

2009 ◽  
Vol 192 (3) ◽  
pp. 834-840 ◽  
Author(s):  
Cheryl M. Vahling ◽  
Yongping Duan ◽  
Hong Lin
Keyword(s):  
Plant Pathogen ◽  
Bioinformatic Analysis ◽  
Intracellular Bacteria ◽  
Cell Competition ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
High Concentrations ◽  
Obligate Intracellular Bacteria

ABSTRACT ATP/ADP translocases transport ATP across a lipid bilayer, which is normally impermeable to this molecule due to its size and charge. These transport proteins appear to be unique to mitochondria, plant plastids, and obligate intracellular bacteria. All bacterial ATP/ADP translocases characterized thus far have been found in endosymbionts of protozoa or pathogens of higher-order animals, including humans. A putative ATP/ADP translocase was uncovered during the genomic sequencing of the intracellular plant pathogen “Candidatus Liberibacter asiaticus,” the causal agent of citrus huanglongbing. Bioinformatic analysis of the protein revealed 12 transmembrane helices and predicted an isoelectric point of 9.4, both of which are characteristic of this family of proteins. The “Ca. Liberibacter asiaticus” gene (nttA) encoding the translocase was subsequently expressed in Escherichia coli and shown to enable E. coli to import ATP directly into the cell. Competition assays with the heterologous E. coli system demonstrated that the translocase was highly specific for ATP and ADP but that other nucleotides, if present in high concentrations, could also be taken up and/or block the ability of the translocase to import ATP. In addition, a protein homologous to NttA was identified in “Ca. Liberibacter solanacearum,” the bacterium associated with potato zebra chip disease. This is the first reported characterization of an ATP translocase from “Ca. Liberibacter asiaticus,” indicating that some intracellular bacteria of plants also have the potential to import ATP directly from their environment.

scholarly journals Molecular Characterization of Carbendazim Resistance of Plant Pathogen (Bipolaris oryzae)

10.5772/29115 ◽  
2011 ◽  
Author(s):  
S. Gomathinayagam ◽  
N. Balasubramanian ◽  
V. Shanmugaiah ◽  
M. Rekha ◽  
P. T. ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Plant Pathogen ◽  
Bipolaris Oryzae

scholarly journals Selection and Characterization of Chitinase-Producing Bacteria Inhibiting the Growth of Plant-Pathogen Fungi

2020 ◽  
Vol 25 (1) ◽  
pp. 35-42
Author(s):  
Azizah Nurmalinda ◽  
◽  
Nisa Rachmania Mubarik ◽  
Lisdar Sudirman ◽  
◽  
...  
Keyword(s):  
Plant Pathogen

scholarly journals The genome sequence of the plant pathogen Xylella fastidiosa

Nature ◽  
2000 ◽  
Vol 406 (6792) ◽  
pp. 151-157 ◽  
Author(s):  
A. J. G. Simpson ◽  
F.C. Reinach ◽  
P. Arruda ◽  
F. A. Abreu ◽  
M. Acencio ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Plant Pathogen ◽  
Xylella Fastidiosa

scholarly journals Natural Infection of Southern Highbush Blueberry (Vaccinium corymbosum Interspecific Hybrids) by Xylella fastidiosa subsp. fastidiosa

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2598-2605
Author(s):  
Dario Di Genova ◽  
Kippy J. Lewis ◽  
Jonathan E. Oliver
Keyword(s):  
Genetic Characterization ◽  
Xylella Fastidiosa ◽  
Natural Infection ◽  
Vaccinium Corymbosum ◽  
Significant Similarity ◽  
Potential Impact ◽  
Southern Highbush ◽  
Southern Georgia ◽  
First Time

Xylella fastidiosa (Xf) is an emerging insect-vectored, xylem-limited bacterium that can cause disease on several economically important fruit and tree crops including almond, blueberry, citrus, grapevine, peach, and pecan. On blueberry, Xf causes bacterial leaf scorch (BLS), which is prevalent in the southeastern United States. This disease, previously reported to be caused by Xf subsp. multiplex (Xfm), can result in rapid plant decline and death of southern highbush (SHB) blueberry cultivars. In 2017, a survey of blueberry plantings in southern Georgia (U.S.A.) confirmed the presence of Xf-infected plants in eight of nine sites examined, and seven isolates were cultured from infected plants. Genetic characterization of these isolates through single-locus and multilocus sequence analysis revealed that three isolates from two sites belonged to Xf subsp. fastidiosa (Xff), with significant similarity to isolates from grapevine. After these three isolates were artificially inoculated onto greenhouse-grown SHB blueberries (cv. ‘Rebel’), symptoms typical of BLS developed, and Xff infection was confirmed through genetic characterization and reisolation of the bacterium to fulfill Koch’s postulates. Because all previously reported Xf isolates from blueberry have been characterized as Xfm, this is the first time that isolation of Xff has been reported from naturally infected blueberry plantings. The potential impact of Xff isolates on disease management in blueberry requires further exploration. Furthermore, given that isolates from both Xfm and Xff were obtained within a single naturally infected blueberry planting, blueberry in southern Georgia may provide opportunities for intersubspecific recombination between Xff and Xfm isolates.

Characterization of the cell wall of the ubiquitous plant pathogen Botrytis cinerea

2009 ◽  
Vol 113 (12) ◽  
pp. 1396-1403 ◽  
Author(s):  
Dario Cantu ◽  
L. Carl Greve ◽  
John M. Labavitch ◽  
Ann L.T. Powell
Keyword(s):  
Cell Wall ◽  
Botrytis Cinerea ◽  
Plant Pathogen
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