scholarly journals Two Divalent Metal Ions and Conformational Changes Play Roles in the Hammerhead Ribozyme Cleavage Reaction

Biochemistry ◽  
2015 ◽  
Vol 54 (41) ◽  
pp. 6369-6381 ◽  
Author(s):  
Aamir Mir ◽  
Ji Chen ◽  
Kyle Robinson ◽  
Emma Lendy ◽  
Jaclyn Goodman ◽  
...  
Keyword(s):  
Metal Ions ◽  
Conformational Changes ◽  
Hammerhead Ribozyme ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Cleavage Reaction

scholarly journals Use of Divalent Metal Ions in the DNA Cleavage Reaction of Human Type II Topoisomerases†

Biochemistry ◽  
2009 ◽  
Vol 48 (9) ◽  
pp. 1862-1869 ◽  
Author(s):  
Joseph E. Deweese ◽  
Amber M. Burch ◽  
Alex B. Burgin ◽  
Neil Osheroff
Keyword(s):  
Metal Ions ◽  
Dna Cleavage ◽  
Divalent Metal ◽  
Type Ii ◽  
Divalent Metal Ions ◽  
Cleavage Reaction ◽  
Human Type

Role of divalent metal ions in the hammerhead RNA cleavage reaction

Biochemistry ◽  
1991 ◽  
Vol 30 (39) ◽  
pp. 9464-9469 ◽  
Author(s):  
SueAnn C. Dahm ◽  
Olke C. Uhlenbeck
Keyword(s):  
Metal Ions ◽  
Divalent Metal ◽  
Rna Cleavage ◽  
Divalent Metal Ions ◽  
Cleavage Reaction ◽  
Hammerhead Rna

pH-Dependent amino acid induced conformational changes of rabbit muscle pyruvate kinase

1980 ◽  
Vol 58 (3) ◽  
pp. 188-193 ◽  
Author(s):  
Chiu-Yin Kwan ◽  
Robert C. Davis
Keyword(s):  
Metal Ions ◽  
Pyruvate Kinase ◽  
Conformational Changes ◽  
Divalent Metal ◽  
Rabbit Muscle ◽  
Divalent Metal Ions ◽  
Spectral Changes ◽  
Metal Derivatives ◽  
Muscle Pyruvate Kinase ◽  
Difference Absorption

The interactions of L-Phe and L-Ala with rabbit muscle pyruvate kinase depended upon the nature of divalent metal ions studied: Mg(II), Co(II), Mn(II), and Ni(II). L-Phe inhibited all metal derivatives of the enzyme except Mn(II)–enzyme. L-Ala inhibited only Ni(II)–enzyme and had no effect on other metal derivatives. The inhibition by L-Phe could be partially or completely reversed by L-Ala for all metal derivatives. The mode of inhibition of pyruvate kinase by L-Phe depended upon pH as well as the nature of activating divalent metal ions. The sigmoidal response increased with increasing pH for all metal derivatives inhibited by L-Phe. L-Phe and L-Ala strongly perturbed the coordination sphere of enzyme bound Co(II), but not Ni(II). There were poor correlations between visible circular dichroic (cd) spectral changes and the corresponding kinetic changes. However, L-Phe and (or) L-Ala induced ultraviolet cd and difference absorption spectral changes, on the other hand, corresponded remarkably well with the kinetic observations.

Local conformational changes in the 8–17 deoxyribozyme core induced by activating and inactivating divalent metal ions

2017 ◽  
Vol 15 (41) ◽  
pp. 8802-8809 ◽  
Author(s):  
Alessio Peracchi ◽  
Maria Bonaccio ◽  
Alfredo Credali
Keyword(s):  
Metal Ions ◽  
Conformational Change ◽  
Conformational Changes ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Specific Metal

Placing 2-aminopurine at position 15 of the 8–17 DNAzyme allows the detection of a specific metal-induced conformational change, apparently coupled to the activation of catalysis.

scholarly journals Use of divalent metal ions in the DNA cleavage reaction of topoisomerase IV

2011 ◽  
Vol 39 (11) ◽  
pp. 4808-4817 ◽  
Author(s):  
S. L. Pitts ◽  
G. F. Liou ◽  
L. A. Mitchenall ◽  
A. B. Burgin ◽  
A. Maxwell ◽  
...  
Keyword(s):  
Metal Ions ◽  
Dna Cleavage ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Cleavage Reaction ◽  
Topoisomerase Iv

Divalent metal ions and the internal equilibrium of the hammerhead ribozyme

Biochemistry ◽  
1995 ◽  
Vol 34 (44) ◽  
pp. 14435-14440 ◽  
Author(s):  
David M. Long ◽  
Frederick J. LaRiviere ◽  
Olke C. Uhlenbeck
Keyword(s):  
Metal Ions ◽  
Hammerhead Ribozyme ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Internal Equilibrium

Interaction of divalent metal ions with the NADP+-malic enzyme from maize leaves

1991 ◽  
Vol 81 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Maria Fabiana Drincovich ◽  
Alberto A. Iglesias ◽  
Carlos S. Andreo
Keyword(s):  
Metal Ions ◽  
Malic Enzyme ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Maize Leaves
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