scholarly journals Spectroscopic Determination of Distinct Heme Ligands in Outer-Membrane Receptors PhuR and HasR ofPseudomonas aeruginosa

Biochemistry ◽  
2015 ◽  
Vol 54 (16) ◽  
pp. 2601-2612 ◽  
Author(s):  
Aaron D. Smith ◽  
Anuja R. Modi ◽  
Shengfang Sun ◽  
John H. Dawson ◽  
Angela Wilks
Keyword(s):  
Outer Membrane ◽  
Membrane Receptors ◽  
Spectroscopic Determination ◽  
Heme Ligands

scholarly journals Cloning and Characterization of an Outer Membrane Protein of Vibrio vulnificus Required for Heme Utilization: Regulation of Expression and Determination of the Gene Sequence

1998 ◽  
Vol 66 (7) ◽  
pp. 3134-3141 ◽  
Author(s):  
Christine M. Litwin ◽  
Burke L. Byrne
Keyword(s):  
Amino Acids ◽  
Outer Membrane ◽  
Vibrio Vulnificus ◽  
Outer Membrane Proteins ◽  
Heme Iron ◽  
Membrane Receptors ◽  
Regulation Of Expression ◽  
Reading Frame

ABSTRACT Vibrio vulnificus is a halophilic, marine pathogen that has been associated with septicemia and serious wound infections in patients with iron overload and preexisting liver disease. ForV. vulnificus, the ability to acquire iron from the host has been shown to correlate with virulence. V. vulnificus is able to use host iron sources such as hemoglobin and heme. We previously constructed a fur mutant ofV. vulnificus which constitutively expresses at least two iron-regulated outer membrane proteins, of 72 and 77 kDa. The N-terminal amino acid sequence of the 77-kDa protein purified from theV. vulnificus fur mutant had 67% homology with the first 15 amino acids of the mature protein of the Vibrio cholerae heme receptor, HutA. In this report, we describe the cloning, DNA sequence, mutagenesis, and analysis of transcriptional regulation of the structural gene for HupA, the heme receptor ofV. vulnificus. DNA sequencing of hupAdemonstrated a single open reading frame of 712 amino acids that was 50% identical and 66% similar to the sequence of V. cholerae HutA and similar to those of other TonB-dependent outer membrane receptors. Primer extension analysis localized one promoter for the V. vulnificus hupAgene. Analysis of the promoter region of V. vulnificus hupA showed a sequence homologous to the consensus Fur box. Northern blot analysis showed that the transcript was strongly regulated by iron. An internal deletion in the V. vulnificus hupA gene, done by using marker exchange, resulted in the loss of expression of the 77-kDa protein and the loss of the ability to use hemin or hemoglobin as a source of iron. ThehupA deletion mutant of V. vulnificus will be helpful in future studies of the role of heme iron in V. vulnificus pathogenesis.

scholarly journals Spectroscopic Determination of an Anion−π Bond Strength

2019 ◽  
Vol 141 (15) ◽  
pp. 6132-6135 ◽  
Author(s):  
Cate S. Anstöter ◽  
Joshua P. Rogers ◽  
Jan R. R. Verlet
Keyword(s):  
Bond Strength ◽  
Spectroscopic Determination

One-photon VUV-MATI and two-photon IR+VUV-MATI spectroscopic determination of oxetane cation ring-puckering vibrations and conformation

2021 ◽  
Vol 154 (5) ◽  
pp. 054308
Author(s):  
Do Won Kang ◽  
Sung Man Park ◽  
Chung Bin Park ◽  
Bong June Sung ◽  
Hong Lae Kim ◽  
...  
Keyword(s):  
Spectroscopic Determination ◽  
Two Photon ◽  
Ring Puckering

Plasmonic Nanosensors for the Determination of Drug Effectiveness on Membrane Receptors

2016 ◽  
Vol 9 (1) ◽  
pp. 218-223 ◽  
Author(s):  
Rubén Ahijado-Guzmán ◽  
Julia Menten ◽  
Janak Prasad ◽  
Christina Lambertz ◽  
Germán Rivas ◽  
...  
Keyword(s):  
Membrane Receptors ◽  
Drug Effectiveness
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