Self-Competitive Inhibition of the Bacteriophage P22 Tailspike Endorhamnosidase by O-Antigen Oligosaccharides

Biochemistry ◽  
2020 ◽  
Vol 59 (51) ◽  
pp. 4845-4855
Author(s):  
Ulrich Baxa ◽  
Andrej Weintraub ◽  
Robert Seckler
Keyword(s):  
Competitive Inhibition ◽  
Bacteriophage P22 ◽  
O Antigen ◽  
P22 Tailspike

scholarly journals Crystal structure of phage P22 tailspike protein complexed with Salmonella sp. O-antigen receptors.

1996 ◽  
Vol 93 (20) ◽  
pp. 10584-10588 ◽  
Author(s):  
S. Steinbacher ◽  
U. Baxa ◽  
S. Miller ◽  
A. Weintraub ◽  
R. Seckler ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Antigen Receptors ◽  
O Antigen ◽  
Phage P22 ◽  
P22 Tailspike ◽  
Tailspike Protein

scholarly journals An essential serotype recognition pocket on phage P22 tailspike protein forces Salmonella enterica serovar Paratyphi A O-antigen fragments to bind as nonsolution conformers

Glycobiology ◽  
2013 ◽  
Vol 23 (4) ◽  
pp. 486-494 ◽  
Author(s):  
Dorothee Andres ◽  
Ulrich Gohlke ◽  
Nina K Broeker ◽  
Stefan Schulze ◽  
Wolfgang Rabsch ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
O Antigen ◽  
Phage P22 ◽  
P22 Tailspike ◽  
Tailspike Protein

TEMPERATURE-SENSITIVE MUTANTS BLOCKED IN THE FOLDING OR SUBUNIT ASSMBLY OF THE BACTERIOPHAGE P22 TAILSPIKE PROTEIN. I. FINE-STRUCTURE MAPPING

Genetics ◽  
1980 ◽  
Vol 96 (2) ◽  
pp. 331-352 ◽  
Author(s):  
Donna H Smith ◽  
Peter B Berget ◽  
Jonathan King
Keyword(s):  
Fine Structure ◽  
Polypeptide Chain ◽  
Cell Attachment ◽  
Physical Map ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Structure Mapping ◽  
Bacteriophage P22 ◽  
P22 Tailspike ◽  
Ts Mutations

ABSTRACT As part of a study of protein folding, we have constructed a fine-structure map of 9 existing and 29 newly isolated UV- and hydroxylamine-induced temperature-sensitive (ts) mutations in gene 9 of Salmonella bacteriophage P22. Gene 9 specifies the polypeptide chain of the multimeric tail spikes, six of which form the cell attachment organelle of the phage. The 38 ts mutants were mapped against deletion lysogens with endpoints in gene 9. They mapped in 10 of the 15 deletion intervals. Two- and three-factor crosses between mutants within each interval indicated that at least 31 ts sites are represented among the 38 mutants. To determine the distribution of ts sites within the physical map, we identified the protein fragments from infection of su- hosts with 10 gene 9 amber mutants. Their molecular weights, ranging from 13,900 to 55,000 daltons, were combined with the genetic data to yield a composite map of gene 9. The 31 ts sites were distributed through most of the gene, but were most densely clustered in the central third.—None of the ts mutant pairs tested exhibited intragenic complementation. Studies of the defective phenotypes of the ts mutants (Goldenberg and King 1981; Smith and King 1981) revealed that most do not affect the thermostability of the mature protein, but instead prevent the folding or subunit assembly of the mutant chains synthesized at restrictive temperature. Thus, many of thes ts mutations identify sites in the polypeptide chain that are critical for the folding or maturation of the tail-spike protein.

Interaction of a Salmonella enteritidis O-antigen octasaccharide with the phage P22 tailspike protein by NMR spectroscopy and docking studies

2007 ◽  
Vol 25 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Jens Landström ◽  
Eva-Lisa Nordmark ◽  
Robert Eklund ◽  
Andrej Weintraub ◽  
Robert Seckler ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Salmonella Enteritidis ◽  
Docking Studies ◽  
O Antigen ◽  
Phage P22 ◽  
P22 Tailspike ◽  
Tailspike Protein

scholarly journals The isolation and sequence of missense and nonsense mutations in the cloned bacteriophage P22 tailspike protein gene.

Genetics ◽  
1989 ◽  
Vol 121 (4) ◽  
pp. 635-649 ◽  
Author(s):  
J J Schwarz ◽  
P B Berget
Keyword(s):  
Dna Sequencing ◽  
Expression Vector ◽  
Bacteriophage P22 ◽  
Nonsense Mutations ◽  
Protein Coding ◽  
Transcription Terminator ◽  
P22 Tailspike ◽  
Cloned Gene ◽  
Tailspike Protein ◽  
New Mutations

Abstract Twenty-seven new mutations in the structural gene for the Salmonella typhimurium bacteriophage P22 tailspike protein have been isolated, mapped using a powerful plasmid-based genetic system and their DNA sequence changes determined. The mutations were generated by hydroxylamine treatment of the cloned gene on a plasmid expression vector. Assaying the activity of the tailspike protein produced from this plasmid and screening for plasmid mutants were accomplished by the in situ complementation of P22 capsids imbedded in soft agar to produce infectious phage. Deletion mutations in the cloned gene have been constructed by a two step procedure involving oligonucleotide linker insertion and in vitro deletion by restriction endonuclease digestion. The deletions, whose physical endpoints were determined by DNA sequencing, define 12 genetic and physical intervals into which the new mutations were mapped by marker rescue experiments. These deletions were transferred to phage P22 by recombination and used to map mutations carried on plasmids. Following mapping, the nucleotide change for each of the mutations was determined by DNA sequencing. The majority were absolute missense mutations although both amber and ochre nonsense mutations were also identified in the protein coding portion of the gene. The suppression pattern of the nonsense mutations was determined on several nonsense suppressors. Four of the mutations cause severely depressed levels of tailspike protein expression from both the cloned gene on the plasmid expression vector and from P22 phage carrying these mutations. These mutations were identified as nucleotide changes in what is probably the P22 late operon transcription terminator which immediately follows the tailspike protein coding sequence.

scholarly journals Equine intestinal O-seroconverting temperate coliphage Hf4s: genomic and biological characterization

2021 ◽  
Author(s):  
Eugene E. Kulikov ◽  
Alla K. Golomidova ◽  
Alexandr D. Efimov ◽  
Ilya S. Belalov ◽  
Maria A. Letarova ◽  
...  
Keyword(s):  
High Density ◽  
Biological Characterization ◽  
Bacteriophage P22 ◽  
E Coli ◽  
O Antigen ◽  
Primary Receptor ◽  
Microbial Ecosystems

Tailed bacteriophages constitute the bulk of the intestinal viromes of vertebrate animals. However, the relationships between lytic and lysogenic lifestyles of phages in these ecosystems are not always clear and may vary between the species or even between the individuals. The human intestinal (fecal) viromes are dominated mostly by temperate phages, while in horse feces virulent phages are more prevalent. Up to our knowledge, all the previously reported isolates of horse fecal coliphages are virulent. Temperate coliphage Hf4s was isolated from horse feces on the indigenous equine E. coli 4s strain. It is a podovirus, related to the Lederbergvirus genus (including the well–characterized Salmonella bacteriophage P22). Hf4s recognizes the host O antigen as its primary receptor and possesses a functional O antigen seroconversion cluster that renders the lysogens protected from superinfection by the same bacteriophage and also abolishes the adsorption of some indigenous equine virulent coliphages, such as DT57C, while other phages, such as G7C or phiKT retain the ability to infect E. coli 4s (Hf4s) lysogens. Importance: The relationships between virulent and temperate bacteriophages and their impact on high-density symbiotic microbial ecosystems of animals are not always clear and may vary between species or even between individuals. The horse intestinal virome is dominated by virulent phages, and Hf4s is the first temperate equine intestinal coliphage characterized. It recognizes the host O antigen as its primary receptor and possesses a functional O antigen seroconversion cluster that renders the lysogens protected from superinfection by some indigenous equine virulent coliphages, such as DT57C, while other phages, such as G7C or phiKT retain the ability to infect E. coli 4s (Hf4s) lysogens. These findings raise questions on the significance of bacteriophage-bacteriophage interactions on the ecology of microbial viruses in mammal intestinal ecosystems.

scholarly journals In vitro Analysis of O-Antigen-Specific Bacteriophage P22 Inactivation by Salmonella Outer Membrane Vesicles

2020 ◽  
Vol 11 ◽  
Author(s):  
Mareike S. Stephan ◽  
Nina K. Broeker ◽  
Athanasios Saragliadis ◽  
Norbert Roos ◽  
Dirk Linke ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Bacteriophage P22 ◽  
O Antigen ◽  
Vitro Analysis ◽  
Specific Bacteriophage ◽  
In Vitro Analysis
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