scholarly journals The NBDY Microprotein Regulates Cellular RNA Decapping

Biochemistry ◽  
2020 ◽  
Vol 59 (42) ◽  
pp. 4131-4142 ◽  
Author(s):  
Zhenkun Na ◽  
Yang Luo ◽  
Jeremy A. Schofield ◽  
Stephanie Smelyansky ◽  
Alexandra Khitun ◽  
...  
Keyword(s):  
Rna Decapping

scholarly journals Structural insights into dpCoA-RNA decapping by NudC

RNA Biology ◽  
2021 ◽  
Author(s):  
Wei Zhou ◽  
Zeyuan Guan ◽  
Fen Zhao ◽  
Yage Ye ◽  
Fang Yang ◽  
...  
Keyword(s):  
Rna Decapping ◽  
Structural Insights

Global deletion of the RNA decapping enzyme Dcp2 postnatally in male mice results in infertility

2020 ◽  
Vol 526 (2) ◽  
pp. 512-518
Author(s):  
Rui-Min Li ◽  
Ming-Nan Zhang ◽  
Qun-Ye Tang ◽  
Man-Gen Song
Keyword(s):  
Male Mice ◽  
Decapping Enzyme ◽  
Rna Decapping

scholarly journals Epigenetic loss of the RNA decapping enzyme NUDT16 mediates C-MYC activation in T-cell acute lymphoblastic leukemia

Leukemia ◽  
2017 ◽  
Vol 31 (7) ◽  
pp. 1622-1625 ◽  
Author(s):  
C Anadón ◽  
G van Tetering ◽  
H J Ferreira ◽  
C Moutinho ◽  
A Martínez-Cardús ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Lymphoblastic Leukemia ◽  
Cell Acute Lymphoblastic Leukemia ◽  
Decapping Enzyme ◽  
Rna Decapping

scholarly journals Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex

RNA ◽  
2016 ◽  
Vol 22 (4) ◽  
pp. 518-529 ◽  
Author(s):  
Marcin Ziemniak ◽  
Jeffrey S. Mugridge ◽  
Joanna Kowalska ◽  
Robert E. Rhoads ◽  
John D. Gross ◽  
...  
Keyword(s):  
Rna Decapping

scholarly journals A Novel NAD-RNA Decapping Pathway Discovered by Synthetic Light-Up NAD-RNAs

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 513
Author(s):  
Florian Abele ◽  
Katharina Höfer ◽  
Patrick Bernhard ◽  
Julia Grawenhoff ◽  
Maximilian Seidel ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Rna Degradation ◽  
Enzymatic Reactions ◽  
Nicotinamide Mononucleotide ◽  
Decapping Enzyme ◽  
New Research ◽  
Rna Decapping ◽  
Decapping Enzymes

The complexity of the transcriptome is governed by the intricate interplay of transcription, RNA processing, translocation, and decay. In eukaryotes, the removal of the 5’-RNA cap is essential for the initiation of RNA degradation. In addition to the canonical 5’-N7-methyl guanosine cap in eukaryotes, the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) was identified as a new 5’-RNA cap structure in prokaryotic and eukaryotic organisms. So far, two classes of NAD-RNA decapping enzymes have been identified, namely Nudix enzymes that liberate nicotinamide mononucleotide (NMN) and DXO-enzymes that remove the entire NAD cap. Herein, we introduce 8-(furan-2-yl)-substituted NAD-capped-RNA (FurNAD-RNA) as a new research tool for the identification and characterization of novel NAD-RNA decapping enzymes. These compounds are found to be suitable for various enzymatic reactions that result in the release of a fluorescence quencher, either nicotinamide (NAM) or nicotinamide mononucleotide (NMN), from the RNA which causes a fluorescence turn-on. FurNAD-RNAs allow for real-time quantification of decapping activity, parallelization, high-throughput screening and identification of novel decapping enzymes in vitro. Using FurNAD-RNAs, we discovered that the eukaryotic glycohydrolase CD38 processes NAD-capped RNA in vitro into ADP-ribose-modified-RNA and nicotinamide and therefore might act as a decapping enzyme in vivo. The existence of multiple pathways suggests that the decapping of NAD-RNA is an important and regulated process in eukaryotes.

Assessing Messenger RNA Decapping in Cellular Extracts

2004 ◽  
pp. 181-192
Author(s):  
Naomi Bergman ◽  
Joseph Milone ◽  
Elizabeth J. Bates ◽  
Mateusz Opyrchal ◽  
Vivian Bellofatto ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Rna Decapping

scholarly journals Induction of RNA interference by C. elegans mitochondrial dysfunction via the DRH-1/RIG-I homologue RNA helicase and the EOL-1/RNA decapping enzyme

2020 ◽  
Author(s):  
Kai Mao ◽  
Peter Breen ◽  
Gary Ruvkun
Keyword(s):  
Rna Interference ◽  
Mitochondrial Dysfunction ◽  
C Elegans ◽  
Antiviral Responses ◽  
Unfolded Protein ◽  
Decapping Enzyme ◽  
Protein Response ◽  
Rna Decapping ◽  
Mitochondrial Unfolded Protein Response ◽  
Transcriptional Induction

AbstractRNA interference (RNAi) is an antiviral pathway common to many eukaryotes that detects and cleaves foreign nucleic acids. In mammals, mitochondrially localized proteins such as MAVS, RIG-I, and MDA5 mediate antiviral responses. Here, we report that mitochondrial dysfunction in Caenorhabditis elegans activates RNAi-directed silencing via induction of a pathway homologous to the mammalian RIG-I helicase viral response pathway. The induction of RNAi also requires the conserved RNA decapping enzyme EOL-1/DXO. The transcriptional induction of eol-1 requires DRH-1 as well as the mitochondrial unfolded protein response (UPRmt). Upon mitochondrial dysfunction, EOL-1 is concentrated into foci that depend on the transcription of mitochondrial RNAs that may form dsRNA, as has been observed in mammalian antiviral responses. The enhanced RNAi triggered by mitochondrial dysfunction contributes to the increase in longevity that is induced by mitochondrial dysfunction.

Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor

2020 ◽  
Author(s):  
Yang Luo ◽  
Jeremy A. Schofield ◽  
Zhenkun Na ◽  
Tanja Hann ◽  
Matthew D. Simon ◽  
...  
Keyword(s):  
Peptide Inhibitor ◽  
Decapping Enzyme ◽  
Rna Decapping

scholarly journals Structural basis of prokaryotic NAD-RNA decapping by NudC

Cell Research ◽  
2016 ◽  
Vol 26 (9) ◽  
pp. 1062-1066 ◽  
Author(s):  
Delin Zhang ◽  
Yexing Liu ◽  
Qiang Wang ◽  
Zeyuan Guan ◽  
Jing Wang ◽  
...  
Keyword(s):  
Structural Basis ◽  
Rna Decapping
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