Heterologous Expression, Purification, and Functional Analysis of the Plasmodium falciparum Phosphatidylinositol 4-Kinase IIIβ

Biochemistry ◽  
2020 ◽  
Vol 59 (27) ◽  
pp. 2494-2506 ◽  
Author(s):  
Anna R. Sternberg ◽  
Paul D. Roepe
Keyword(s):  
Functional Analysis ◽  
Plasmodium Falciparum ◽  
Heterologous Expression ◽  
Phosphatidylinositol 4

Heterologous Expression, Purification, and Functional Analysis of Plasmodium falciparum Phosphatidylinositol 3′-Kinase

Biochemistry ◽  
2017 ◽  
Vol 56 (33) ◽  
pp. 4335-4345 ◽  
Author(s):  
Matthew R. Hassett ◽  
Anna R. Sternberg ◽  
Bryce E. Riegel ◽  
Craig J. Thomas ◽  
Paul D. Roepe
Keyword(s):  
Functional Analysis ◽  
Plasmodium Falciparum ◽  
Heterologous Expression ◽  
Phosphatidylinositol 3 Kinase ◽  
Phosphatidylinositol 3

scholarly journals Using Yeast Synthetic Lethality To Inform Drug Combination for Malaria

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Suvitha Subramaniam ◽  
Christoph D. Schmid ◽  
Xue Li Guan ◽  
Pascal Mäser
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Combination ◽  
Synthetic Lethality ◽  
Drug Combinations ◽  
Synthetic Lethal Interaction ◽  
Content Type ◽  
Synthetic Lethal ◽  
Gene Pairs ◽  
Phosphatidylinositol 4

ABSTRACT Combinatorial chemotherapy is necessary for the treatment of malaria. However, finding a suitable partner drug for a new candidate is challenging. Here we develop an algorithm that identifies all of the gene pairs of Plasmodium falciparum that possess orthologues in yeast that have a synthetic lethal interaction but are absent in humans. This suggests new options for drug combinations, particularly for inhibitors of targets such as P. falciparum calcineurin, cation ATPase 4, or phosphatidylinositol 4-kinase.

A unique phosphatidylinositol 4-phosphate 5-kinase is activated by ADP-ribosylation factor in Plasmodium falciparum

2009 ◽  
Vol 39 (6) ◽  
pp. 645-653 ◽  
Author(s):  
Werner Leber ◽  
Alison Skippen ◽  
Quinton L. Fivelman ◽  
Paul W. Bowyer ◽  
Shamshad Cockcroft ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Adp Ribosylation ◽  
Phosphatidylinositol 4 ◽  
Adp Ribosylation Factor

Heterologous expression of proteins from Plasmodium falciparum: Results from 1000 genes

2006 ◽  
Vol 148 (2) ◽  
pp. 144-160 ◽  
Author(s):  
Christopher Mehlin ◽  
Erica Boni ◽  
Frederick S. Buckner ◽  
Linnea Engel ◽  
Tiffany Feist ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Heterologous Expression

scholarly journals Saquinavir Inhibits the Malaria Parasite's Chloroquine Resistance Transporter

2012 ◽  
Vol 56 (5) ◽  
pp. 2283-2289 ◽  
Author(s):  
Rowena E. Martin ◽  
Alice S. Butterworth ◽  
Donald L. Gardiner ◽  
Kiaran Kirk ◽  
James S. McCarthy ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Heterologous Expression ◽  
Protease Inhibitors ◽  
Chloroquine Resistance ◽  
Content Type ◽  
Chloroquine Resistance Transporter ◽  
Resistant Phenotype ◽  
Transgenic Parasites

ABSTRACTThe antiretroviral protease inhibitors (APIs) ritonavir, saquinavir, and lopinavir, used to treat HIV infection, inhibit the growth ofPlasmodium falciparumat clinically relevant concentrations. Moreover, it has been reported that these APIs potentiate the activity of chloroquine (CQ) against this parasitein vitro. The mechanism underlying this effect is not understood, but the degree of chemosensitization varies between the different APIs and, with the exception of ritonavir, appears to be dependent on the parasite exhibiting a CQ-resistant phenotype. Here we report a study of the role of theP. falciparumchloroquine resistance transporter (PfCRT) in the interaction between CQ and APIs, using transgenic parasites expressing different PfCRT alleles and using theXenopus laevisoocyte system for the heterologous expression of PfCRT. Our data demonstrate that saquinavir behaves as a CQ resistance reverser and that this explains, at least in part, its ability to enhance the effects of CQ in CQ-resistantP. falciparumparasites.

Isoform Characterisation, Heterologous Expression and Functional Analysis of Two Lectins from Vatairea macrocarpa

2013 ◽  
Vol 20 (11) ◽  
pp. 1204-1210 ◽  
Author(s):  
João Alves Filho ◽  
Antonia Fernandes do Nascimento ◽  
Ana Silva Gondim ◽  
Ronniery Pereira ◽  
Rodrigo Silva da Cunha ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Heterologous Expression ◽  
Vatairea Macrocarpa
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