Sensitive Detection of Transcription Factor in Nuclear Extracts by Target-Actuated Isothermal Amplification-Mediated Fluorescence Enhancement

2017 ◽  
Vol 89 (19) ◽  
pp. 10439-10445 ◽  
Author(s):  
Yan Zhang ◽  
Dongxue Xiang ◽  
Bo Tang ◽  
Chun-yang Zhang
Keyword(s):  
Transcription Factor ◽  
Fluorescence Enhancement ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Nuclear Extracts

Sensitive Detection of Transcription Factor by Coupled Fluorescence-encoded Microsphere with Exonuclease Protection

Talanta ◽  
2021 ◽  
pp. 122272
Author(s):  
Yue Sun ◽  
Liu Zang ◽  
Choiwan Lau ◽  
Xueji Zhang ◽  
Jianzhong Lu
Keyword(s):  
Transcription Factor ◽  
Sensitive Detection

scholarly journals Rapid and sensitive detection of Nosema bombycis using loop-mediated isothermal amplification and colorimetric nanogold

ScienceAsia ◽  
2019 ◽  
Vol 45 (3) ◽  
pp. 268
Author(s):  
Weijiang Dai ◽  
Jingru Qi ◽  
Hongli Chen ◽  
Zhilin Zhang ◽  
Ruisha Shang ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Nosema Bombycis ◽  
Loop Mediated Isothermal Amplification

Two transcriptional activators, CCAAT-box-binding transcription factor and heat shock transcription factor, interact with a human hsp70 gene promoter

1987 ◽  
Vol 7 (3) ◽  
pp. 1129-1138
Author(s):  
W D Morgan ◽  
G T Williams ◽  
R I Morimoto ◽  
J Greene ◽  
R E Kingston ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Gene Promoter ◽  
Heat Shock Transcription Factor ◽  
Transcriptional Activators ◽  
Hsp70 Gene ◽  
Nuclear Extracts ◽  
Ccaat Box ◽  
Human Hsp70

We characterized the activity of a human hsp70 gene promoter by in vitro transcription. Analysis of 5' deletion and substitution mutants in HeLa nuclear extracts showed that the basal activity of the promoter depends primarily on a CCAAT-box sequence located at -65. A protein factor, CCAAT-box-binding transcription factor (CTF), was isolated from HeLa nuclear extracts and shown to be responsible for stimulation of transcription in a reconstituted in vitro system. DNase I footprinting revealed that CTF interacts with two CCAAT-box elements located at -65 and -147 of the human hsp70 promoter. An additional binding activity, heat shock transcription factor (HSTF), which interacted with the heat shock element, was also identified in HeLa extract fractions. This demonstrates that the promoter of this human hsp70 gene interacts with at least two positive transcriptional activators, CTF, which is required for CCAAT-box-dependent transcription as in other promoters such as those of globin and herpes simplex virus thymidine kinase genes, and HSTF, which is involved in heat inducibility.

Identification of promoter elements necessary for transcriptional regulation of a human histone H4 gene in vitro

1985 ◽  
Vol 5 (2) ◽  
pp. 380-389
Author(s):  
S M Hanly ◽  
G C Bleecker ◽  
N Heintz
Keyword(s):  
Transcription Factor ◽  
Transcription Initiation ◽  
Initiation Site ◽  
S Phase ◽  
Histone H4 ◽  
Promoter Elements ◽  
Nuclear Extracts ◽  
Distal Promoter ◽  
Histone H4 Gene

We have examined the nucleotide sequences necessary for transcription of a human histone H4 gene in vitro. Maximal transcription of the H4 promoter requires, in addition to the TATA box and cap site, promoter elements between 70 and 110 nucleotides upstream from the transcription initiation site. These distal promoter elements are recognized preferentially in extracts from synchronized S-phase HeLa cells. The inability of non-S-phase nuclear extracts to recognize the H4 upstream sequences reflects a specific lack of a transcription factor which interacts with those sequences. These results indicate that the cell cycle regulation of human histone gene expression involves both a specific transcription factor and distal transcription signals in the H4 promoter.

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