A Peptide Cleavage-Based Ultrasensitive Electrochemical Biosensor with an Ingenious Two-Stage DNA Template for Highly Efficient DNA Exponential Amplification

2017 ◽  
Vol 89 (17) ◽  
pp. 8951-8956 ◽  
Author(s):  
Ding Wang ◽  
Yaqin Chai ◽  
Yali Yuan ◽  
Ruo Yuan
Keyword(s):  
Electrochemical Biosensor ◽  
Two Stage ◽  
Peptide Cleavage ◽  
Highly Efficient ◽  
Dna Template

scholarly journals Development of a Highly Efficient SOFC Module Using Two-stage Stacks and a Fuel Regeneration Process

Fuel Cells ◽  
2017 ◽  
Vol 17 (4) ◽  
pp. 535-540 ◽  
Author(s):  
K. Nakamura ◽  
T. Ide ◽  
S. Taku ◽  
T. Nakajima ◽  
M. Shirai ◽  
...  
Keyword(s):  
Regeneration Process ◽  
Two Stage ◽  
Highly Efficient

scholarly journals An electrochemical biosensor for rapid detection of E. coli O157:H7 with highly efficient bi-functional glucose oxidase-polydopamine nanocomposites and Prussian blue modified screen-printed interdigitated electrodes

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5441-5449 ◽  
Author(s):  
Meng Xu ◽  
Ronghui Wang ◽  
Yanbin Li
Keyword(s):  
Prussian Blue ◽  
Glucose Oxidase ◽  
Rapid Detection ◽  
Electrochemical Biosensor ◽  
Interdigitated Electrodes ◽  
E Coli ◽  
Highly Efficient ◽  
Screen Printed

An electrochemical biosensor was developed based on the bifunctional ABs/GOxext/AuNPs/MBs-GOx@PDA magnetic PMNCs that can rapidly and sensitively detect E. coli O157:H7.

Peptide cleavage-based electrochemical biosensor coupling graphene oxide and silver nanoparticles

2019 ◽  
Vol 1047 ◽  
pp. 45-51 ◽  
Author(s):  
Fanyu Meng ◽  
Haixuan Sun ◽  
Yue Huang ◽  
Yuguo Tang ◽  
Qiang Chen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Electrochemical Biosensor ◽  
Peptide Cleavage

The Two-Stage Recognition Method Based on Texture Signals of the Heterogeneous Unsteady Iris

2021 ◽  
Author(s):  
Shuai Liu ◽  
Yuanning Liu ◽  
Xiaodong Zhu ◽  
Jing Liu ◽  
Guang Huo ◽  
...  
Keyword(s):  
Iris Recognition ◽  
Recognition Accuracy ◽  
Texture Features ◽  
Unsteady State ◽  
Recognition Method ◽  
Two Stage ◽  
Second Stage ◽  
Highly Efficient ◽  
Training Samples ◽  
Gray Values

In this paper, a two-stage multi-category recognition structure based on texture features is proposed. This method can solve the problem of the decline in recognition accuracy in the scene of lightweight training samples. Besides, the problem of recognition effect different in the same recognition structure caused by the unsteady iris can also be solved. In this paper’s structure, digitized values of the edge shape in the iris texture of the image are set as the texture trend feature, while the differences between the gray values of the image obtained by convolution are set as the grayscale difference feature. Furthermore, the texture trend feature is used in the first-stage recognition. The template category that does not match the tested iris is the elimination category, and the remaining categories are uncertain categories. Whereas, in the second-stage recognition, uncertain categories are adopted to determine the iris recognition conclusion through the grayscale difference feature. Then, the experiment results using the JLU iris library show that the method in this paper can be highly efficient in multi-category heterogeneous iris recognition under lightweight training samples and unsteady state.

Highly efficient production of FAMEs and β-farnesene from a two-stage biotransformation of waste cooking oils

2019 ◽  
Vol 199 ◽  
pp. 112001 ◽  
Author(s):  
Jiajia Lv ◽  
Yuefei Wang ◽  
Chengyu Zhang ◽  
Shengping You ◽  
Wei Qi ◽  
...  
Keyword(s):  
Efficient Production ◽  
Two Stage ◽  
Highly Efficient ◽  
Waste Cooking Oils ◽  
Cooking Oils

A highly efficient two-stage cultivation strategy for lutein production using heterotrophic culture of Chlorella sorokiniana MB-1-M12

2018 ◽  
Vol 253 ◽  
pp. 141-147 ◽  
Author(s):  
Chun-Yen Chen ◽  
I-Chia Lu ◽  
Dillirani Nagarajan ◽  
Chien-Hsiang Chang ◽  
I-Son Ng ◽  
...  
Keyword(s):  
Chlorella Sorokiniana ◽  
Heterotrophic Culture ◽  
Two Stage ◽  
Highly Efficient ◽  
Cultivation Strategy ◽  
Lutein Production

Cl-assisted highly efficient synthesis of FePd3 alloys encapsulated in graphite papers: a two stage CVD approach

RSC Advances ◽  
2016 ◽  
Vol 6 (47) ◽  
pp. 40676-40682 ◽  
Author(s):  
Jian Guo ◽  
Qingyu Ye ◽  
Mu Lan ◽  
Shanling Wang ◽  
Tian Yu ◽  
...  
Keyword(s):  
Efficient Synthesis ◽  
Two Stage ◽  
Cvd Method ◽  
Highly Efficient

We propose an advanced two-stage CVD method which allows the synthesis of very thick deposits of planar rolled-like graphite structures filled with FePd3 alloys as dominant product in the entire reactor.

scholarly journals WAYS TO INCREASE THE PRODUCTIVITY IN CONTINUOUSLY OPERATING TRENCHING EXCAVATORS

2021 ◽  
Vol 1 (50) ◽  
pp. 132-143
Author(s):  
Musiiko V ◽  
◽  
Koval A ◽  
Patsora D ◽  
◽  
...  
Keyword(s):  
Machine Building ◽  
Discharge Process ◽  
Two Stage ◽  
Study Objective ◽  
Highly Efficient ◽  
Scientific Hypothesis ◽  
Efficient Construction ◽  
Soil Flow ◽  
The One ◽  
Soil Excavation

The article considers and reasons the ways and possibilities to create the highly efficient constructions of trenching excavators that dig trenches of the defined profile by means of using bucketless rotors as soil excavating implements together with design of the discharge assembly improved construction. Study objective: to create a bucketless highly efficient rotary implement of the trenching excavator with two-stage discharge. Study object: continuously operation earthmoving machines. Research technique – analytical-experimental. One of the most important problems for the machine-building industry of Ukraine is the efficiency improvement of machines created in the country. Solving of this problem is required due to necessity to perform the increasing amount of earthworks in building and construction works. The cheapest way to perform lengthy soil excavations is to excavate them with trenching excavators. Increasing of their productivity is the one of the main directions to improve the efficiency of the earthworks mechanization. Increasing the efficiency of trenching excavators is possible by means bucketless rotary implement usage and by improving of the rotary discharge process. The study justifies and proves the scientific hypothesis about the possibility to use the force of the soil flow header pressure create by the soil that is being transported from the excavation for the bucketless rotary implements discharge. This allows by means of two-stage discharge of the implement to ensure the full clean-up and to improve the productivity of the soil excavation. The study analytically justifies the requirements for the main construction parameters of the bucketless rotor. Based on the obtained results the technical proposal is created for highly efficient construction of the bucketless rotary implement of the trenching excavator with two-stage discharge. KEYWORDS: BUCKETLESS ROTOR, SOIL, TWO-STAGE, DISCHARGE, DISCHARGE ASSEMBLY, TRENCH DIGGER

scholarly journals Synthesis of Libraries and Multi-Site Mutagenesis using a PCR derived, dU-containing Template

2021 ◽  
Author(s):  
Gretchen Meinke ◽  
Nahide Dalda ◽  
Benjamin S Brigham ◽  
Andrew Bohm
Keyword(s):  
Genetic Diversity ◽  
Target Sequence ◽  
Low Background ◽  
Oligonucleotide Primers ◽  
Highly Efficient ◽  
Dna Libraries ◽  
Site Mutagenesis ◽  
Product Sequence ◽  
Dna Template ◽  
Single Reaction

Abstract Directed DNA libraries are useful because they focus genetic diversity in the most important regions within a sequence. Ideally, all sequences in such libraries should appear with the same frequency and there should be no significant background from the starting sequence. These properties maximize the number of different sequences that can be screened. Described herein is a method termed SLUPT (Synthesis of Libraries via a dU-containing PCR Template) for generating highly targeted DNA libraries and/or multi-site mutations wherein the altered bases may be widely distributed within a target sequence. This method is highly efficient and modular. Moreover, multiple distinct sites, each with one or more base changes, can be altered in a single reaction. There is very low background from the starting sequence, and SLUPT libraries have similar representation of each base at the positions selected for variation. The SLUPT method utilizes a single stranded dU-containing DNA template that is made by PCR. Synthesis of the template in this way is significantly easier than has been described earlier. A series of oligonucleotide primers that are homologous to the template and encode the desired genetic diversity are extended and ligated in a single reaction to form the mutated product sequence or library. After selective inactivation of the template, only the product library is amplified. There are no restrictions on the spacing of the mutagenic primers except that they cannot overlap.

Highly Efficient, Full ZVS, Hybrid, Multilevel DC/DC Topology for Two-Stage Grid-Connected 1500-V PV System With Employed 900-V SiC Devices

2019 ◽  
Vol 7 (2) ◽  
pp. 811-832 ◽  
Author(s):  
Branislav Stevanovic ◽  
Diego Serrano ◽  
Miroslav Vasic ◽  
Pedro Alou ◽  
Jesus Angel Oliver ◽  
...  
Keyword(s):  
Pv System ◽  
Two Stage ◽  
Highly Efficient
Sign in / Sign up

Export Citation Format

Share Document