scholarly journals Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells

2017 ◽  
Vol 89 (2) ◽  
pp. 1138-1146 ◽  
Author(s):  
Ling Hao ◽  
Jillian Johnson ◽  
Christopher B. Lietz ◽  
Amanda Buchberger ◽  
Dustin Frost ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Quantitative Proteomics ◽  
Mass Defect ◽  
Pancreatic Cancer Cells

Abstract 4533: Mechanism of anti-proliferative effects of sanguinarine in pancreatic cancer cells: A label-free quantitative proteomics approach

2014 ◽  
Author(s):  
Chandra K. Singh ◽  
Satwinderjeet Kaur ◽  
Jasmine George ◽  
Molly C. Pellitteri-Hahn ◽  
Cameron O. Scarlett ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Quantitative Proteomics ◽  
Label Free ◽  
Pancreatic Cancer Cells ◽  
Proteomics Approach

scholarly journals SILAC-Based Quantitative Proteomics Identifies Multifactorial Mechanism of Oxaliplatin Resistance in Pancreatic Cancer Cells

2020 ◽  
Author(s):  
Young Eun Kim ◽  
Eun-Kyung Kim ◽  
Min-Jeong Song ◽  
Tae-Young Kim ◽  
Ho Hee Jang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Quantitative Proteomics ◽  
Cellular Mechanisms ◽  
Stable Isotope Labelling ◽  
Kinase Substrate ◽  
Pancreatic Cancer Cells ◽  
Interferon Signalling ◽  
New Strategies

AbstractOxaliplatin is a commonly used chemotherapeutic drug for the treatment of pancreatic cancer. Understanding the cellular mechanisms of oxaliplatin resistance is important for developing new strategies to overcome drug resistance in pancreatic cancer. In this study, we performed a stable isotope labelling by amino acids in cell culture (SILAC)-based quantitative proteomic analysis of oxaliplatin-resistant and sensitive pancreatic cancer PANC-1 cells. We identified 107 proteins whose expression levels changed between oxaliplatin-resistant and sensitive cells, which were involved in multiple biological processes, including DNA repair, drug response, apoptotic signalling, and the type 1 interferon signalling pathway. Notably, myristoylated alanine-rich C-kinase substrate (MARCKS) and wntless homolog protein (WLS) were upregulated in oxaliplatin-resistant cells compared to sensitive cells, as confirmed by qRT-PCR and Western blot analysis. We further demonstrated the activation of AKT and β-catenin signalling (downstream targets of MARCKS and WLS, respectively) in oxaliplatin-resistant PANC-1 cells. Additionally, we show that the siRNA-mediated suppression of both MARCKS and WLS enhanced oxaliplatin sensitivity in oxaliplatin-resistant PANC-1 cells. Taken together, our results provide insights into multiple mechanisms of oxaliplatin resistance in pancreatic cancer cells and reveal that MARCKS and WLS might be involved in the chemotherapeutic resistance in pancreatic cancer.

scholarly journals Quantitative Proteomics: Measuring Protein Synthesis Using15N Amino Acid Labeling in Pancreatic Cancer Cells

2009 ◽  
Vol 81 (2) ◽  
pp. 764-771 ◽  
Author(s):  
Yingchun Zhao ◽  
Wai-Nang Paul Lee ◽  
Shu Lim ◽  
Vay Liang Go ◽  
Jing Xiao ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Cancer Cells ◽  
Quantitative Proteomics ◽  
Pancreatic Cancer Cells ◽  
Amino Acid Labeling
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