Multifunctional Gold Nanoclusters-Based Nanosurface Energy Transfer Probe for Real-Time Monitoring of Cell Apoptosis and Self-Evaluating of Pro-Apoptotic Theranostics

2016 ◽  
Vol 88 (22) ◽  
pp. 11184-11192 ◽  
Author(s):  
Yong Li ◽  
Pei Li ◽  
Rong Zhu ◽  
Chao Luo ◽  
Hao Li ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Cell Apoptosis ◽  
Gold Nanoclusters ◽  
Real Time Monitoring

Real-time monitoring and control of CHO cell apoptosis by in situ multifrequency scanning dielectric spectroscopy

2019 ◽  
Vol 80 ◽  
pp. 138-145 ◽  
Author(s):  
Fuduo Ma ◽  
An Zhang ◽  
David Chang ◽  
Orlin D. Velev ◽  
Kelly Wiltberger ◽  
...  
Keyword(s):  
Real Time ◽  
Dielectric Spectroscopy ◽  
Cell Apoptosis ◽  
Monitoring And Control ◽  
Real Time Monitoring ◽  
Cho Cell ◽  
And Control

Viscosity-sensitive thiolated gold nanoclusters with diffusion-controlled emission for intracellular viscosity imaging

The Analyst ◽  
2019 ◽  
Vol 144 (15) ◽  
pp. 4483-4487 ◽  
Author(s):  
Saifei Pan ◽  
Jin Zhou ◽  
Weidong Liu ◽  
Yuxin Ye ◽  
Guilin Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Gold Nanoclusters ◽  
Real Time Monitoring ◽  
Diffusion Controlled ◽  
Emission Behavior ◽  
Viscosity Variation

A unique diffusion-controlled emission behavior of gold nanoclusters was reported and further used in real-time monitoring and imaging of intracellular viscosity variation.

scholarly journals Rapid, Homogeneous Genotyping of the 4G/5G Polymorphism in the Promoter Region of the PAI1 Gene by Fluorescence Resonance Energy Transfer and Probe Melting Curves

1999 ◽  
Vol 45 (8) ◽  
pp. 1141-1147 ◽  
Author(s):  
Markus Nauck ◽  
Heinrich Wieland ◽  
Winfried März
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Promoter Region ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Plasminogen Activator Inhibitor ◽  
Real Time Monitoring ◽  
Plasminogen Activator Inhibitor 1 ◽  
Amplification Process ◽  
Pai 1

Abstract Background: Many studies have convincingly shown that survivors of myocardial infarction have impaired fibrinolytic activity because of increased concentrations of plasma plasminogen activator inhibitor-1 (PAI-1). A single guanosine insertion/deletion polymorphism in the promoter region of the PAI1 gene, commonly called 4G/5G, has been shown to be associated with plasma PAI-1 activity. Our aim was to develop and validate a homogeneous assay for rapid genotyping of the 4G/5G polymorphism. Methods: In this report we present a single-tube method for genotyping of the 4G/5G polymorphism that combines both rapid-cycle PCR with real-time monitoring of the amplification process and generation of allele-specific fluorescent probe melting profiles on the LightCyclerTM. Two fluorescently labeled hybridization probes recognizing adjacent sequences in the amplicon were present in the reaction mixture. The shorter detection probe spanned the polymorphic site, perfectly matching the 5G allele. After annealing, the fluorophores were in resonance energy transfer, providing real-time monitoring of the amplification process. At the completion of the PCR, fluorescence was monitored as the temperature increased through the Tm of the probe/product duplex, and a characteristic melting profile for each genotype was obtained. Results: With this method, 32 samples were genotyped within 30 min without the need of any post-PCR sample manipulation. The genotypes of 100 DNA samples determined with the LightCycler were identical to those obtained with conventional PCR and restriction fragment length analysis. Conclusion: The genotyping of the 4G/5G polymorphism with the LightCycler is a rapid, reliable method that is suitable for typing both small and large numbers of samples.

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