Surface-Enhanced Raman Spectroscopy for Identification of Heavy Metal Arsenic(V)-Mediated Enhancing Effect on Antibiotic Resistance

2016 ◽  
Vol 88 (6) ◽  
pp. 3164-3170 ◽  
Author(s):  
Li Cui ◽  
Ying-Jiao Zhang ◽  
Wei E. Huang ◽  
Bi-Feng Zhang ◽  
Francis L. Martin ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Raman Spectroscopy ◽  
Antibiotic Resistance ◽  
Surface Enhanced Raman Spectroscopy ◽  
Enhancing Effect ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Facile Ag-Film Based Surface Enhanced Raman Spectroscopy Using DNA Molecular Switch for Ultra-Sensitive Mercury Ions Detection

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 596 ◽  
Author(s):  
Xiujie Liu ◽  
Mengmeng Liu ◽  
Yudong Lu ◽  
Changji Wu ◽  
Yunchao Xu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Raman Spectroscopy ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Molecular Switch ◽  
Mercury Ions ◽  
Promising Alternative ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Ag Film

Heavy metal pollution has long been the focus of attention because of its serious threat to human health and the environment. Surface enhanced Raman spectroscopy (SERS) has shown great potential for metal detection owing to many advantages, including, requiring fewer samples, its minimal damage to specimen, and its high sensitivity. In this work, we proposed a simple and distinctive method, based on SERS, using facile silver film (Ag-film) combined with a DNA molecular switch, which allowed for the highly specific detection of heavy metal mercury ions (Hg2+). When in the presence of Hg2+ ions, the signals from Raman probes attach to single-stranded DNA, which will be dramatically enhanced due to the specific structural change of DNA strands—resulting from the interaction between Hg2+ ions and DNA bases. This SERS sensor could achieve an ultralow limit of detection (1.35 × 10−15 M) for Hg2+ detection. In addition, we applied this SERS sensor to detect Hg2+ in real blood samples. The results suggested that this SERS platform could be a promising alternative tool for Hg2+ detection in clinical, environmental, and food inspection.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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