Using Molecular Beacons To Probe Molecular Interactions between Lactate Dehydrogenase and Single-Stranded DNA

2000 ◽  
Vol 72 (14) ◽  
pp. 3280-3285 ◽  
Author(s):  
Xiaohong Fang ◽  
Jianwei Jeff Li ◽  
Weihong Tan
Keyword(s):  
Lactate Dehydrogenase ◽  
Molecular Interactions ◽  
Molecular Beacons ◽  
Single Stranded Dna

scholarly journals Molecular Interactions of a DNA Modifying Enzyme APOBEC3F Catalytic Domain with a Single-Stranded DNA

2018 ◽  
Vol 430 (1) ◽  
pp. 87-101 ◽  
Author(s):  
Yao Fang ◽  
Xiao Xiao ◽  
Shu-Xing Li ◽  
Aaron Wolfe ◽  
Xiaojiang S. Chen
Keyword(s):  
Molecular Interactions ◽  
Catalytic Domain ◽  
Single Stranded Dna

Galloflavin prevents the binding of lactate dehydrogenase A to single stranded DNA and inhibits RNA synthesis in cultured cells

2013 ◽  
Vol 430 (2) ◽  
pp. 466-469 ◽  
Author(s):  
Luigi Fiume ◽  
Marina Vettraino ◽  
Domenica Carnicelli ◽  
Valentina Arfilli ◽  
Giuseppina Di Stefano ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Rna Synthesis ◽  
Cultured Cells ◽  
Single Stranded Dna

scholarly journals Identification of the mouse low-salt-eluting single-stranded DNA-binding protein as a mammalian lactate dehydrogenase-A isoenzyme

1986 ◽  
Vol 233 (3) ◽  
pp. 913-916 ◽  
Author(s):  
F S Sharief ◽  
S H Wilson ◽  
S S-L Li
Keyword(s):  
Lactate Dehydrogenase ◽  
Binding Protein ◽  
Rabbit Antiserum ◽  
Single Stranded Dna ◽  
Ssdna Binding ◽  
Low Salt ◽  
Ssdna Binding Protein ◽  
A Subunit ◽  
Cellulose Column ◽  
Mouse Myeloma

A 36,000-Mr protein purified from mouse myeloma on the basis of selective binding to a single-stranded DNA (ssDNA)-cellulose column has been identified as the lactate dehydrogenase A (LDH-A) subunit. A homogeneous preparation of this mouse myeloma ssDNA-binding protein, termed the ‘low-salt-eluting protein’, was found to possess LDH activity, and rabbit antiserum prepared against this protein was shown to cross-react with purified 36,000-Mr LDH-A subunits from mouse and bovine sources. In addition, bovine and human LHD-A4 isoenzymes were shown to be capable of binding ssDNA. These enzymic and immunological identities with LDH-A were not observed with purified helix-destabilizing protein 1 from mouse myeloma. A model for ssDNA-LDH binding is discussed.

A major single-stranded DNA binding protein from ovaries of the frog, Xenopus laevis, is lactate dehydrogenase

1989 ◽  
Vol 1008 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Howard B. Kaiserman ◽  
Ward F. Odenwald ◽  
Deborah J. Stowers ◽  
Everdina H.A. Poll ◽  
Robert M. Benbow
Keyword(s):  
Lactate Dehydrogenase ◽  
Dna Binding ◽  
Xenopus Laevis ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna

Dynamics and molecular interactions of single-stranded DNA in nucleic acid biosensors with varied surface properties

2019 ◽  
Vol 21 (29) ◽  
pp. 16367-16380 ◽  
Author(s):  
Timothy Cholko ◽  
Shivansh Kaushik ◽  
Chia-en A. Chang
Keyword(s):  
Nucleic Acid ◽  
Surface Properties ◽  
Molecular Interactions ◽  
Dynamic Properties ◽  
Self Assembled Monolayers ◽  
Single Stranded Dna ◽  
Assembled Monolayers ◽  
Self Assembled

Simulations reveal the dynamic properties of freely diffusing and surface-tethered ssDNA on self-assembled monolayers with widely varying surface properties.

Dual color fluorescence quantitative detection of specific single-stranded DNA with molecular beacons and nucleic acid dye SYBR Green I

The Analyst ◽  
2012 ◽  
Vol 137 (16) ◽  
pp. 3787 ◽  
Author(s):  
Dong-Shan Xiang ◽  
Guo-Hua Zhou ◽  
Ming Luo ◽  
Xing-Hu Ji ◽  
Zhi-Ke He
Keyword(s):  
Nucleic Acid ◽  
Sybr Green ◽  
Molecular Beacons ◽  
Sybr Green I ◽  
Quantitative Detection ◽  
Acid Dye ◽  
Single Stranded Dna ◽  
Dual Color

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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