Characteristics of Different Nucleic Acid Staining Dyes for DNA Fragment Sizing by Flow Cytometry

1999 ◽  
Vol 71 (24) ◽  
pp. 5470-5480 ◽  
Author(s):  
Xiaomei Yan ◽  
W. Kevin Grace ◽  
Thomas M. Yoshida ◽  
Robert C. Habbersett ◽  
Nileena Velappan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Dna Fragment

Development of a Mechanism-Based, DNA Staining Protocol Using SYTOX Orange Nucleic Acid Stain and DNA Fragment Sizing Flow Cytometry

2000 ◽  
Vol 286 (1) ◽  
pp. 138-148 ◽  
Author(s):  
Xiaomei Yan ◽  
Robert C. Habbersett ◽  
Julia M. Cordek ◽  
John P. Nolan ◽  
Thomas M. Yoshida ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Dna Staining ◽  
Staining Protocol ◽  
Dna Fragment ◽  
Nucleic Acid Stain

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

scholarly journals Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry

2002 ◽  
Vol 28 ◽  
pp. 131-140 ◽  
Author(s):  
P Lebaron ◽  
P Servais ◽  
AC Baudoux ◽  
M Bourrain ◽  
C Courties ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Cell Size ◽  
Acid Content ◽  
Specific Activity ◽  
Nucleic Acid Content

Flow Cytometry to EvaluateAnaplasma marginaleParasitemia Using a Fluorescent Nucleic Acid Stain

2008 ◽  
Vol 1149 (1) ◽  
pp. 111-113 ◽  
Author(s):  
Rosalía Moretta ◽  
Paula Ruybal ◽  
María Mesplet ◽  
Romina Petrigh ◽  
Pablo Nuñez ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Nucleic Acid Stain

Single Molecule Nucleic Acid Analysis by Fluorescence Flow Cytometry

2002 ◽  
pp. 351-370
Author(s):  
Peter M. Goodwin ◽  
W. Patrick Ambrose ◽  
Hong Cai ◽  
W. Kevin Grace ◽  
Erica J. Larson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Single Molecule ◽  
Nucleic Acid Analysis

Flow cytometry for rapid size determination and sorting of nucleic acid containing nanoparticles in biological fluids

2010 ◽  
Vol 141 (3) ◽  
pp. 328-338 ◽  
Author(s):  
E.V.B. van Gaal ◽  
G. Spierenburg ◽  
W.E. Hennink ◽  
D.J.A. Crommelin ◽  
E. Mastrobattista
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Biological Fluids ◽  
Size Determination

scholarly journals Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2062-2074 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
High Throughput ◽  
Double Emulsion ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Acid Recovery ◽  
Emulsion Flow ◽  
Double Emulsion Droplets

We have developed a novel workflow (sdDE-FACS, s̲ingle d̲roplet D̲ouble E̲mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.

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