Analysis for TNF-α Using Solid-Phase Affinity Capture with Radiolabel and MALDI-MS Detection

1999 ◽  
Vol 71 (20) ◽  
pp. 4727-4733 ◽  
Author(s):  
Gregory B. Hurst ◽  
Michelle V. Buchanan ◽  
Linda J. Foote ◽  
Stephen J. Kennel
Keyword(s):  
Solid Phase ◽  
Maldi Ms ◽  
Affinity Capture ◽  
Ms Detection ◽  
Tnf Α

scholarly journals Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

Lab on a Chip ◽  
2013 ◽  
Vol 13 (11) ◽  
pp. 2055-2065 ◽  
Author(s):  
Iulia M. Lazar ◽  
Jarod L. Kabulski
Keyword(s):  
Maldi Ms ◽  
Ms Detection ◽  
Sample Extraction ◽  
On Chip

Determination of 3,3'-Dichlorobenzidine in Industrial Wastewaters

2004 ◽  
Vol 39 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Hing-Biu Lee ◽  
Thomas E. Peart ◽  
Ken Terry ◽  
James Maguire
Keyword(s):  
Electron Impact ◽  
Chemical Ionization ◽  
Solid Phase ◽  
Negative Ion ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Detection ◽  
Negative Ion Chemical Ionization ◽  
Ionization Mode ◽  
Wastewater Samples

Abstract A solid-phase extraction, chemical derivatization, and gas chromatography/mass spectrometry (GC/MS) method has been developed for the determination of trace 3,3'-dichlorobenzidine (DCB) in industrial wastewater samples. Instead of an octadecylsilane (ODS) cartridge, a Porapak RDX cartridge was used since the latter could be optimized for the extraction of DCB. After the pre-concentration, interfering coextractives in the sample were selectively removed from the cartridge by elution with a 1:1 mixture of acetonitrile and water. DCB was then eluted with acetonitrile and derivatized with pentafluoropropionic acid anhydride. The product was subsequently analyzed by GC/MS in either the electron impact or negative ion chemical ionization mode. Recoveries of DCB and the surrogate, DCB-d6, were better than 90% at spiking levels of 10, 1, and 0.1 µg/L. Based on a concentration factor of 100, the detection limits for DCB in wastewater samples were determined to be 0.1 µg/L by electron impact GC/MS detection, and 0.01 µg/L by negative ion chemical ionization GC/MS detection, respectively. This method has been successfully applied to wastewater samples collected in the Toronto area.

Solid-phase methylamidation for sialoglycomics by MALDI-MS

2014 ◽  
Vol 406 (25) ◽  
pp. 6235-6246 ◽  
Author(s):  
Henghui Li ◽  
Xing Zhao ◽  
Qiwei Zhang ◽  
Xiaojun Feng ◽  
Bi-Feng Liu ◽  
...  
Keyword(s):  
Solid Phase ◽  
Maldi Ms

scholarly journals THE CONTENT OF SERUM CYTOTOXINS UNDER LIVER CIRRHOSIS OF VIRAL ETIOLOGY AT VARIOUS STAGES OF COMPENSATION

2018 ◽  
Vol 24 (2) ◽  
pp. 83-86
Author(s):  
Vladimir Evgenievich Kulikov ◽  
M. G Sharafutdinov ◽  
M. E Khapman ◽  
M. A Toneeva ◽  
K. V Nikolaeva ◽  
...  
Keyword(s):  
Reference Values ◽  
Solid Phase ◽  
Portal Pressure ◽  
Average Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Etiology ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Class C ◽  
The Given

The study was implemented concerning the level of interleukins 2 and 6 (IL-2 and IL-6), tumor necrosis factor alpha (TNF-α) under hepatic cirrhosis (HC) of viral etiology classes А, В, С according Child-Pugh. The purpose was to evaluate level of serum cytokinemies and their relationship under HC of viral etiology depending on class of severity according Child-Pugh and level of portal pressure. The cytokines concentration was determined using technique of solid-phase enzyme-linked immunosorbent assay. It is established that against the background of decreasing of compensation of HC occurs increasing of serum level of IL-2, IL-6 and TNF-α within the limits of reference values at the exception of level of IL-6 under HC class C. In the given category of patients, level of IL-6 exceeds reference values and is within the limits of 9,94-25,21 pg ̸ml with average concentration 14,89±4,96 pg ̸ml. It is demonstrated that quantitative ratios and degrees of relationships between cytokines are determined by compensation of cirrhosis. The correlation relationships are mostly expressed in the form of feedback between TNF-α and IL-6 (r = 0,499) in case of HC class A. and in the form of direct relation between TNF-α and IL-2 (r = 0,421) in case of HC class B. It is proved that against the background of decreasing of compensation of HC increasing is occurring of serum levels of IL-2, IL-6 and TNF-α within the limits of reference values with the exception of level of IL-6 in case of HC class C. In the given category of patients’ level of IL-6 exceeds reference values and is within limits of 9,94-25,21 pg/ml with average concentration 14,89 4,96 pg/ml.

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