Capillary Electrochromatography with Novel Stationary Phases. 3. Retention Behavior of Small and Large Nucleic Acids on Octadecyl-Sulfonated-Silica

1999 ◽  
Vol 71 (15) ◽  
pp. 3277-3282 ◽  
Author(s):  
Minquan Zhang ◽  
Changming Yang ◽  
Ziad El Rassi
Keyword(s):  
Nucleic Acids ◽  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Retention Behavior ◽  
Sulfonated Silica

Recent progress in adsorbed stationary phases for capillary electrochromatography

2002 ◽  
Vol 23 (22-23) ◽  
pp. 3954-3972 ◽  
Author(s):  
Zhen Liu ◽  
Ren'an Wu ◽  
Hanfa Zou
Keyword(s):  
Capillary Electrochromatography ◽  
Recent Progress ◽  
Stationary Phases

Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

2017 ◽  
Vol 100 (6) ◽  
pp. 1652-1659 ◽  
Author(s):  
Anna Petruczynik ◽  
Justyna Misiurek ◽  
Tomasz Tuzimski ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Ionic Liquid ◽  
Stationary Phases ◽  
Reversed Phase ◽  
System Efficiency ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Retention Behavior ◽  
Isoquinoline Alkaloids ◽  
Mobile Phases ◽  
Chemical Groups

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

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