Investigation of Solid-Phase Peptide Synthesis by the Near-Infrared Multispectral Imaging Technique:  A Detection Method for Combinatorial Chemistry

1999 ◽  
Vol 71 (13) ◽  
pp. 2255-2261 ◽  
Author(s):  
Marc Fischer ◽  
Chieu D. Tran
Keyword(s):  
Peptide Synthesis ◽  
Combinatorial Chemistry ◽  
Near Infrared ◽  
Multispectral Imaging ◽  
Detection Method ◽  
Solid Phase ◽  
Imaging Technique ◽  
Solid Phase Peptide Synthesis

Visualising Chemical Composition and Reaction Kinetics by the near Infrared Multispectral Imaging Technique

2000 ◽  
Vol 8 (2) ◽  
pp. 87-99 ◽  
Author(s):  
Chieu D. Tran
Keyword(s):  
Peptide Synthesis ◽  
Near Infrared ◽  
Multispectral Imaging ◽  
Solid Phase ◽  
Imaging Technique ◽  
Solid Phase Peptide Synthesis ◽  
Imaging Spectrometer ◽  
Kinetic Determination ◽  
Nir Imaging ◽  
Single Pixel

A new multispectral imaging spectrometer which is capable of simultaneously recording spectral images in the visible and near infrared has been developed. In this instrument, an acousto-optic tunable filter (AOTF) is used to diffract an unpolarised incident light into two diffracted beams with orthogonal polarisation. One of the beams is detected by a silicon camera for the visible region while the other is detected in the near infrared (NIR) region (from 1000 to 1700nm) with an NIR camera. The imaging spectrometer is sensitive, inexpensive and field deployable because it is based on a camera using the recently available InGaAs focal plane arrays which are low-cost and can be sensitively operated at room temperature. The imaging spectrometer was used for measurements which previously were not possible. These include the kinetic determination of curing of an epoxy resin by amine, and the detection of reaction products from solid-phase peptide synthesis. Rates of reactions between epoxy and amine were found to be very inhomogeneous. Because of this kinetic inhomogeneity, differences in the degrees of cure at different positions within the sample can be as high as 37% when data from only a single pixel were used for calculation. The inhomogeneity was not observed if the average of a large number of pixels was used. The NIR imaging spectrometer was also used for the kinetic determination and identification of products formed during the solid phase peptide synthesis of glycine, alanine and valine mediated by aminomethylstyrene resin beads. Because this NIR imaging spectrometer can measure spectra at different positions within a sample, it was used for the first demonstration in which reactions of three different solid-phase peptide syntheses (in a three-compartment cell) were simultaneously monitored. Since relatively good spectra can be obtained by using data recorded by a single pixel and because the NIR camera has 240×320 pixels, this NIR multispectral imaging technique is not limited to three-compartment cell used in this study but rather can be used as the detection method for solid-phase peptide synthesis in combinatorial chemistry.

A Strategy for Thioamide Incorporation During Fmoc Solid-Phase Peptide Synthesis with Robust Stereochemical Integrity

2019 ◽  
Author(s):  
luis camacho III ◽  
Bryan J. Lampkin ◽  
Brett VanVeller
Keyword(s):  
Amino Acid ◽  
Functional Groups ◽  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Side Chains ◽  
Amino Acid Side Chains ◽  
Peptide Elongation

We describe a method to protect the sensitive stereochemistry of the thioamide—in analogy to the protection of the functional groups of amino acid side chains—in order to preserve the thioamide moiety during peptide elongation.<br>

Analytical Methods for Solid Phase Peptide Synthesis

2004 ◽  
Vol 8 (4) ◽  
pp. 291-301 ◽  
Author(s):  
Giuseppina Sabatino ◽  
Mario Chelli ◽  
Alberto Brandi ◽  
Anna Papini
Keyword(s):  
Peptide Synthesis ◽  
Analytical Methods ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis

Fmoc Solid Phase Peptide Synthesis

1999 ◽  
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Peptide Chain ◽  
Standard Approach ◽  
Side Chain ◽  
Complex Structures ◽  
Protein Conjugation ◽  
Chemoselective Ligation ◽  
Routine Production

In the years since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The basic problems outstanding at the time of publication of this earlier work have now been, for the most part, solved. As a result, innovators in the field have focussed their efforts to develop methodologies and chemistry for the synthesis of more complex structures. The focus of this new volume is much broader, and covers not only the essential procedures for the production of linear peptides but also more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods also deserving attention have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is also covered, as well as methods for overcoming aggregation during peptide chain assembly and a survey of available automated instrumentation.

scholarly journals Cover Picture: Propylphosphonic Anhydride (T3P®) as Coupling Reagent for Solid‐Phase Peptide Synthesis (11/2021)

2021 ◽  
Vol 6 (11) ◽  
pp. 2648-2648
Author(s):  
Othman Al Musaimi ◽  
Richard Wisdom ◽  
Peter Talbiersky ◽  
Beatriz G. De La Torre ◽  
Fernando Albericio
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Coupling Reagent

Synthesis of MUC1 Peptide and Glycopeptide Dendrimers

2009 ◽  
Vol 62 (10) ◽  
pp. 1339 ◽  
Author(s):  
Candy K. Y. Chun ◽  
Richard J. Payne
Keyword(s):  
Peptide Synthesis ◽  
Tandem Repeat ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Cycloaddition Reaction ◽  
Repeat Sequence ◽  
Tandem Repeat Sequence ◽  
Multiple Copies ◽  
Fmoc Strategy

Several dendrimers possessing multiple copies of peptides and glycopeptides belonging to the MUC1 eicosapeptide tandem repeat sequence have been prepared. Fmoc-strategy solid-phase peptide synthesis was used to construct the peptides and glycopeptides, which were conjugated to suitably functionalized dendrimer cores using the copper-catalyzed azide-alkyne cycloaddition reaction to produce multivalent peptide and glycopeptide dendrimers.

Synthesis of 2-hydroxyethylsulfonyl-methyl-substituted polystyrenes and their application in solid phase peptide synthesis

Tetrahedron ◽  
1976 ◽  
Vol 32 (9) ◽  
pp. 1069-1071 ◽  
Author(s):  
Godefridus I. Tesser ◽  
Jan T.W.A.R.M. Buis ◽  
Erik Th.M. Wolters ◽  
Elizabeth G.A.M. Bothé-Helmes
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis
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