High-Throughput Bioanalytical LC/MS/MS Determination of Benzodiazepines in Human Urine:  1000 Samples per 12 Hours

1999 ◽  
Vol 71 (13) ◽  
pp. 2294-2300 ◽  
Author(s):  
Jerry Zweigenbaum ◽  
Katja Heinig ◽  
Simon Steinborner ◽  
Timothy Wachs ◽  
Jack Henion
Keyword(s):  
High Throughput ◽  
Human Urine

scholarly journals Comparison of an HPLC-MS/MS Method with Multiple Commercial ELISA Kits on the Determination of Levels of 8-oxo-7,8-Dihydro-2'-Deoxyguanosine in Human Urine

2018 ◽  
Vol 2 (2) ◽  
pp. 1-13 ◽  
Author(s):  
Kun-Ming Chen ◽  
Ana Calcagnotto ◽  
Junjia Zhu ◽  
Yuan-Wan Sun ◽  
Karam El-Bayoumy ◽  
...  
Keyword(s):  
High Throughput ◽  
Human Urine ◽  
Low Cost ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Immunochemical Methods ◽  
Spe Purification

Introduction: Analysis of 8-oxodG is usually conducted by either chromatography-based methods or by immunochemical methods commonly used based upon their low cost and high-throughput. However, concern regarding the accuracy of ELISA methods has complicated their use. We directly compare the levels of urinary 8-oxodG obtained by HPLC-MS/MS with three commercially available ELISA kits in this report. Methods: In the current study, a total of 9 human urine samples were analyzed by LC-MS/MS and three commonly used commercial available ELISA kits. Results: We found that urinary 8-oxodG levels analyzed by HPLC-MS/MS [1.4 ± 0.3 nmol/mmol creatinine) were 7.6- to 23.5-fold lower than those detected by ELISA. Overall, the correlations between ELISA and HPLC-MS/MS were poor but were improved after SPE purification for kits from ENZO (P = 0.2817 without SPE; P = 0.0086 with SPE) and Abcam (P = 0.0596 without SPE; P = 0.0473 with SPE). Discussion and conclusion: While we confirmed that SPE purification can improve the correlation between the selected ELISA kits and HPLC-MS/MS, HPLC-MS/MS is still the method of choice to accurately assess the levels of 8-oxodG in human urine.

A CHEMICAL METHOD FOR THE DETERMINATION OF 2-HYDROXYOESTRONE IN HUMAN URINE

1973 ◽  
Vol 71 (4_Suppl) ◽  
pp. S110
Author(s):  
H. P. Gelbke ◽  
R. Knuppen
Keyword(s):  
Human Urine ◽  
Chemical Method
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