Isolation of Priority Polycyclic Aromatic Hydrocarbons from Natural Sediments and Sludge Reference Materials by an Anti-Fluorene Immunosorbent Followed by Liquid Chromatography and Diode Array Detection

1998 ◽  
Vol 70 (23) ◽  
pp. 4996-5001 ◽  
Author(s):  
Sandra Pérez ◽  
Imma Ferrer ◽  
Marie-Claire Hennion ◽  
Damià Barceló
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Reference Materials ◽  
Aromatic Hydrocarbons ◽  
Diode Array ◽  
Diode Array Detection ◽  
Polycyclic Aromatic ◽  
Array Detection

Determination of Polycyclic Aromatic Hydrocarbons in Commercial Parenteral Formulations and Medications Using High-Performance Liquid Chromatography with Diode Array Detection

2017 ◽  
Vol 100 (4) ◽  
pp. 1070-1076 ◽  
Author(s):  
Marcia M Barichello ◽  
Denise Bohrer ◽  
Carine Viana ◽  
Leandro M Carvalho ◽  
Paulo C Nascimento
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Pharmaceutical Products ◽  
Diode Array ◽  
Diode Array Detection ◽  
Polycyclic Aromatic ◽  
Array Detection ◽  
Method Accuracy

Abstract HPLC coupled to UV diode array detection (DAD) is proposed for the determination of polycyclic aromatic hydrocarbons (PAHs) in pharmaceutical products for parenteral administration. Because rubber is a possible source of PAHs for these products, samples stored in containers with rubber parts were selected for the analysis. The basis for method optimization was EPA Method 8310, which determines 16 priority PAHs in ground water and wastewater by HPLC using both UV and fluorescence detection. Using DAD, two channels were selected for detection, with one operating at 254 nm for the detection of nine PAHs and the other at 225 nm for the detection of seven PAHs. This method allowed for the detection of PAHs using external calibration with LODs and LOQs ranging from 0.001 to 0.060 µg/mL and from 0.003 to 0.167 µg/mL, respectively. Within-day precision, expressed as RSD, varied from 1.24 to 7.76% for PAH concentrations from 0.05 to 0.50 µg/mL, and intraday precision varied from 3.10 to 9.40% for the same concentration range. Method accuracy was confirmed by recoveries of 75–120% of the spiked samples. This method was applied for the determination of PAHs in three commercial infusion solutions and in nine different medications stored in syringes prior to administration to patients. Twelve of 16 PAHs were found in these samples. Total PAH concentrations varied from 0.13 to 13.50 µg/mL. Pyrene was the most prevalent contaminant, being present in 11 of 12 samples in concentrations ranging from 0.17 to 4.80 µg/mL. This method presented good sensitivity for the measurement of PAH in the target samples, allowing for the determination of the 16 priority PAHs in one run and in 30 min.

Collection of polycyclic aromatic sulfur heterocycles from asphalt fumes and quantification by HPLC-DAD method

2021 ◽  
Author(s):  
Rejane Secretti Cargnin ◽  
Franciele Rovasi Adolfo ◽  
Paulo Cicero Nascimento ◽  
Patrícia Schmidt ◽  
Letícia Callegaro Brudi ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Diode Array ◽  
Diode Array Detection ◽  
Polycyclic Aromatic Sulfur Heterocycles ◽  
Polycyclic Aromatic ◽  
Sulfur Heterocycles ◽  
Array Detection ◽  
Asphalt Fumes

A chromatographic method is described for the separation and quantification of polycyclic aromatic sulfur heterocycles (PASHs) using liquid chromatography coupled with diode array detection (DAD). The PASHs that were investigated...

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