General Protease Assay Method Coupling Solid-Phase Substrate Extraction and Capillary Electrophoresis

1998 ◽  
Vol 70 (18) ◽  
pp. 3824-3827 ◽  
Author(s):  
Douglas B. Craig ◽  
Jerome C. Y. Wong ◽  
Robert Polakowski ◽  
Norman J. Dovichi
Keyword(s):  
Capillary Electrophoresis ◽  
Solid Phase ◽  
Assay Method ◽  
Protease Assay

Bioanalytical method development and validation of simultaneous analysis of telmisartan and hydrochlorthiazide in human plasma using LC-MS/MS

2016 ◽  
Vol 5 (03) ◽  
pp. 4862 ◽  
Author(s):  
Mathew George* ◽  
Lincy Joseph ◽  
Arpit Kumar Jain ◽  
Anju V.
Keyword(s):  
Human Plasma ◽  
Retention Time ◽  
High Performance ◽  
Method Development ◽  
Matrix Effects ◽  
Solid Phase ◽  
Internal Standard ◽  
Assay Method ◽  
Buffer System ◽  
Internal Standards

A simple, sensitive, rapid and economic high performance thin layer chromatographic method and a mass spectroscopic assay method has been developed for the quantification of telmisartan and hydrochlorthiazide combination in human plasma. The internal standards and analytes were extracted from human plasma by solid-phase extraction with HLB Oasis1cc (30mg) catridges. The scanning and optimization for the samples are done using methanol: water (50:50). The samples were chromatographed using reverse phase chromatography with C-18 column of different manufacturers like Ascentis C18 (150×4. 6, 5µ) using the buffer system Acetonitrile: Buffer (80:20%v/v) which consist of 2±0. 1Mm ammonium format at a flow rate of 0. 7ml/min at a column oven temperature 35±10c. The internal standard used was hydrochlorthiazide13c1, d2 and telmisartand3. The extraction techniques include conditioning, loading, washing and elution, drying followed by reconstitution of the dried samples. The volume injected was 10µl with the retention time of 3-4 min for telmisartan, 1-2 min for hydrochlorthiazide and for the internal standards the retention time was 3-4 min for telmisartand3 and 1-2 min for hydrochlorthiazide c13d2. The rinsing solution was Acetonitrile: HPLC grade water in the ratio (50:50). The above developed method was validated using various parameters like selectivity and sensitivity, accuracy and precision, matrix effects, % recovery and various stability studies. The method was proved to be sensitive, accurate, precise and reproducible. The preparation showed high recovery for the quantitative determination of telmisartan and hydrochlorthiazide in human plasma.

Developments in coupled solid-phase extraction-capillary electrophoresis 2013-2015

2015 ◽  
Vol 37 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Rawi Ramautar ◽  
Govert W. Somsen ◽  
Gerhardus J. de Jong
Keyword(s):  
Capillary Electrophoresis ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction

Ultrasound-assisted magnetic solid-phase extraction for the determination of some transition metals in Orujo spirit samples by capillary electrophoresis

2016 ◽  
Vol 190 ◽  
pp. 263-269 ◽  
Author(s):  
Rosa M. Peña Crecente ◽  
Carlha Gutiérrez Lovera ◽  
Julia Barciela García ◽  
Carlos Herrero Latorre ◽  
Sagrario García Martín
Keyword(s):  
Capillary Electrophoresis ◽  
Transition Metals ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Magnetic Solid ◽  
Ultrasound Assisted

scholarly journals Dispersive solid-phase extraction based on butylamide silica for the determination of sulfamethoxazole in milk samples by capillary electrophoresis

2016 ◽  
Vol 39 (14) ◽  
pp. 658-665 ◽  
Author(s):  
Gabriela Islas ◽  
José A. Rodríguez ◽  
M. Elena Páez-Hernández ◽  
Silvia Corona-Avendaño ◽  
Alberto Rojas-Hernández ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Milk Samples
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