Higher Order Equilibria and Their Effect on Analyte Migration Behavior in Capillary Electrophoresis

1998 ◽  
Vol 70 (15) ◽  
pp. 3261-3270 ◽  
Author(s):  
Michael T. Bowser ◽  
David D. Y. Chen
Keyword(s):  
Capillary Electrophoresis ◽  
Higher Order ◽  
Migration Behavior ◽  
Analyte Migration

Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives

1997 ◽  
Vol 18 (5) ◽  
pp. 706-716 ◽  
Author(s):  
Xuejun Peng ◽  
Michael T. Bowser ◽  
Philip Britz-McKibbin ◽  
Gwendolyn M. Bebault ◽  
Jamie R. Morris ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Description ◽  
Migration Behavior ◽  
Analyte Migration ◽  
Behavior Based

The effects of a mixture of charged and neutral additives on analyte migration behavior in capillary electrophoresis

1998 ◽  
Vol 19 (3) ◽  
pp. 388-396 ◽  
Author(s):  
Andrea R. Kranack ◽  
Michael T. Bowser ◽  
Philip Britz-Mckibbin ◽  
David D. Y. Chen
Keyword(s):  
Capillary Electrophoresis ◽  
Migration Behavior ◽  
Analyte Migration

Quantitative description of analyte migration behavior based on the dynamic complexation model in capillary electrophoresis

1997 ◽  
Vol 75 (5) ◽  
pp. 507-517 ◽  
Author(s):  
Xuejun Peng ◽  
Gwendolyn M. Bebault ◽  
David D.Y. Chen ◽  
Stephen L. Sacks
Keyword(s):  
Capillary Electrophoresis ◽  
Equilibrium Constant ◽  
Electrophoretic Mobility ◽  
Method Development ◽  
Background Electrolyte ◽  
Phase System ◽  
Migration Behavior ◽  
Additive Concentration ◽  
Résolution Équation ◽  
Analyte Migration

A theory based on dynamic complexation is used to describe analyte migration behavior in capillary electrophoresis (CE). This theory is based on a one-phase system, instead of the commonly accepted two-phase system. The migration behavior of an analyte is described by three parameters (the electrophoretic mobility of the free analyte, the electrophoretic mobility of the analyte–additive complex, and the equilibrium constant (formation constant) that determines the fractions of the free analyte and the complex at a certain additive concentration). Varying the additive concentration shifts the equilibrium and changes the viscosity of the background electrolyte. Viscosity correction is crucial in interpreting the observed migration behavior of analytes. While electroosmotic flow in a capillary often varies from one capillary to another, the viscosity of a buffer is characteristic of the buffer composition and is constant for each buffer. The electrophoretic mobility of a certain species and the equilibrium constant are intrinsic properties and are less sensitive to changes in the environment. Understanding these relationships is indispensable in CE method development and method validation. A universal resolution equation is proposed, with a separation factor that has taken both the electrophoretic mobilities and equilibria into consideration. This resolution equation gives clear guidance for the optimization of CE separations. A group of nucleosides and their phosphates are used as analytes, and β-cyclodextrin is used as the additive in the model system studied in this paper. Both the observed analyte migration behavior and the resolution of analytes agree well with this theory. Keywords: dynamic complexation capillary electrophoresis, nucleoside and nucleotide separation, capacity factor, resolution equation, viscosity correction.

Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

2017 ◽  
Vol 31 (16-19) ◽  
pp. 1744094 ◽  
Author(s):  
Chenchen Liu ◽  
Yoshinori Yamaguchi ◽  
Xiaoming Dou
Keyword(s):  
Capillary Electrophoresis ◽  
Small Rna ◽  
Polymer Concentration ◽  
Migration Time ◽  
Migration Behavior ◽  
Molecular Weights ◽  
Rna Fragments ◽  
Molecular Sieving

In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments ([Formula: see text]1000 nt) was improved with the increase of polymer concentration, whereas the large ones ([Formula: see text]3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.

Modeling the migration behavior of rabbit liver apothioneins in capillary electrophoresis

2008 ◽  
pp. NA-NA
Author(s):  
Fernando Benavente ◽  
Balbina Andón ◽  
Estela Giménez ◽  
José Barbosa ◽  
Victoria Sanz-Nebot
Keyword(s):  
Capillary Electrophoresis ◽  
Rabbit Liver ◽  
Migration Behavior

scholarly journals Migration Behavior of Alkylphenols, Bisphenol A and Bisphenol S Studied by Capillary Electrophoresis Using Sulfated .BETA.-Cyclodextrin.

2001 ◽  
Vol 17 (6) ◽  
pp. 763-768 ◽  
Author(s):  
Masanobu MORI ◽  
Hisako NARAOKA ◽  
Hirohito TSUE ◽  
Tatsuya MOROZUMI ◽  
Takashi KANETA ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Bisphenol A ◽  
Migration Behavior ◽  
Bisphenol S ◽  
Beta Cyclodextrin

Migration behavior and selectivity of sulfonamides in capillary electrophoresis

1997 ◽  
Vol 792 (1-2) ◽  
pp. 37-47 ◽  
Author(s):  
Ching-Erh Lin ◽  
Wei-Chen Lin ◽  
Yung-Chih Chen ◽  
Shi-Wei Wang
Keyword(s):  
Capillary Electrophoresis ◽  
Migration Behavior
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