Monitoring Protein Refolding Induced by Disulfide Formation Using Capillary Isoelectric Focusing-Electrospray Ionization Mass Spectrometry

1998 ◽  
Vol 70 (10) ◽  
pp. 2044-2049 ◽  
Author(s):  
Pamela K. Jensen ◽  
A. Kamel Harrata ◽  
Cheng S. Lee
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Protein Refolding ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Disulfide Formation

On-line capillary isoelectric focusing hyphenated to native electrospray ionization mass spectrometry for the characterization of interferon-γ and variants

The Analyst ◽  
2015 ◽  
Vol 140 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Cédric Przybylski ◽  
Meriem Mokaddem ◽  
Mehdi Prull-Janssen ◽  
Els Saesen ◽  
Hugues Lortat-Jacob ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Interferon Γ ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Detection Mode ◽  
On Line

The on-line hyphenation of Capillary IsoElectric Focusing (CIEF) with ElectroSpray Ionization Mass Spectrometry (ESI/MS) has been carried out in a non-denaturing detection mode at the CIEF-MS interface.

Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6928-6936 ◽  
Author(s):  
Liang Chen ◽  
Joan M. Cabot ◽  
Estrella Sanz Rodriguez ◽  
Alireza Ghiasvand ◽  
Peter C. Innis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Electrospray Ionisation ◽  
Ionisation Mass Spectrometry ◽  
Ionisation Mass

Efficient ‘on-thread’ isoelectric focusing of proteins, with direct on-thread detection using desorption electrospray ionisation mass spectrometry.

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