Evaluation of a Sheath Flow Cuvette for Postcolumn Fluorescence Derivatization of DNA Fragments Separated by Capillary Electrophoresis

1998 ◽  
Vol 70 (1) ◽  
pp. 182-186 ◽  
Author(s):  
William F. Nirode ◽  
Tracy D. Staller ◽  
Roderic O. Cole ◽  
Michael J. Sepaniak
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Fragments ◽  
Fluorescence Derivatization ◽  
Sheath Flow ◽  
Sheath Flow Cuvette

Sheath-Flow Cuvette for High-Sensitivity Laser-Induced Fluorescence Detection in Capillary Electrophoresis

2007 ◽  
Vol 61 (7) ◽  
pp. 777-779 ◽  
Author(s):  
Kimia Sobhani ◽  
David A. Michels ◽  
Norman J. Dovichi
Keyword(s):  
Stainless Steel ◽  
Capillary Electrophoresis ◽  
Carbonic Anhydrase ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Flow Chamber ◽  
Fluorescence Detector ◽  
Sheath Flow ◽  
Laser Induced Fluorescence Detection ◽  
Sheath Flow Cuvette

The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3σ) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.

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