Exploiting the Interaction of Metal Ions and Peptide Nucleic Acids−DNA Duplexes for the Detection of a Single Nucleotide Mismatch by Electrochemical Impedance Spectroscopy

2010 ◽  
Vol 82 (3) ◽  
pp. 1166-1169 ◽  
Author(s):  
Congjuan Li ◽  
Xiaohong Li ◽  
Xinhui Liu ◽  
Heinz-Bernhard Kraatz
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Nucleic Acids ◽  
Metal Ions ◽  
Electrochemical Impedance ◽  
Peptide Nucleic Acids ◽  
Dna Duplexes ◽  
Single Nucleotide ◽  
Nucleotide Mismatch ◽  
Single Nucleotide Mismatch

scholarly journals Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jessica Attia ◽  
Sivan Nir ◽  
Evgeniy Mervinetsky ◽  
Dora Balogh ◽  
Agata Gitlin-Domagalska ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Metal Ions ◽  
Conformational Change ◽  
Metal Ion ◽  
Electrochemical Impedance ◽  
Morphological Changes ◽  
Ion Binding ◽  
Host Layer

AbstractPeptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a conformational change of the oxytocin peptide in the monolayer, which was measured using electrochemical impedance spectroscopy. Here, we demonstrate that sensing can be achieved also when the oxytocin is non-covalently integrated into an alkanethiol host monolayer. We show that ion-binding cause morphological changes to the dense host layer, which translates into enhanced impedimetric signals compared to direct covalent assembly strategies. This biosensor proved selective and sensitive for Zn2+ ions in the range of nano- to micro-molar concentrations. This strategy offers an approach to utilize peptide flexibility in monitoring their response to the environment while embedded in a hydrophobic monolayer.

Unlabeled Hairpin-DNA Probe for the Detection of Single-Nucleotide Mismatches by Electrochemical Impedance Spectroscopy

2008 ◽  
Vol 80 (6) ◽  
pp. 2255-2260 ◽  
Author(s):  
Ying Wang ◽  
Congjuan Li ◽  
Xiaohong Li ◽  
Yongfang Li ◽  
Heinz-Bernhard Kraatz
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Dna Probe ◽  
Single Nucleotide ◽  
Hairpin Dna
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