Assessing Differentiation Status of Human Embryonic Stem Cells Noninvasively Using Raman Microspectroscopy

2010 ◽  
Vol 82 (12) ◽  
pp. 5020-5027 ◽  
Author(s):  
H. Georg Schulze ◽  
Stanislav O. Konorov ◽  
Nicolas J. Caron ◽  
James M. Piret ◽  
Michael W. Blades ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Differentiation Status

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Absolute Quantification of Intracellular Glycogen Content in Human Embryonic Stem Cells with Raman Microspectroscopy

2011 ◽  
Vol 83 (16) ◽  
pp. 6254-6258 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
Chad G. Atkins ◽  
James M. Piret ◽  
Samuel A. Aparicio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Glycogen Content ◽  
Embryonic Stem ◽  
Absolute Quantification ◽  
Raman Microspectroscopy

scholarly journals 176 IMPROVED GROWTH OF HUMAN EMBRYONIC STEM CELLS IN A REDUCED OXYGEN ATMOSPHERE

2005 ◽  
Vol 17 (2) ◽  
pp. 238 ◽  
Author(s):  
T. Peura ◽  
A. Bosman ◽  
T. Stojanov
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Culture Conditions ◽  
Oxygen Atmosphere ◽  
Low Oxygen ◽  
Treatment Groups ◽  
Absolute Growth ◽  
Differentiation Status

Traditional cell culture conditions entail the use of gas atmosphere consisting of 5–6% CO2 in air. These same conditions have also been used universally for the culture of human embryonic stem cells (hES), despite the natural milieu of the embryos, from which these cells are derived, being slightly hypoxic. The aim of this work was to examine if human embryonic stem cells would benefit from the reduced oxygen culture environment, as used for human pre-implantation embryos. In Experiment 1, the relative growth in high and low oxygen atmosphere was compared by cutting undifferentiared hES-colonies into equal-size pieces and transferring them to two dishes, one into the conventional incubator in 6% CO2 in air (HIGH), the other into K-MINC-1000 (Eight Mile Plains, Queensland) mini-incubator in 5% O2, 6% CO2 and 89% N2 (LOW). After 8 days the colony sizes and differentiation status were measured. In Experiment 2, the absolute growth in high and low oxygen atmosphere was compared by cutting exactly same size fragments from undifferentiated hES-colonies and distributing them to two treatments as described. All colonies were measured and passaged at Day 7 and final measurements taken at Day 14. In Experiment 1, improved growth was observed in reduced oxygen, mean percentage of undifferentiated growth from original colony (assigned as 100%) being 217% for HIGH and 482% for LOW over three replicates. In Experiment 2, the total of 16.9 mm2 of undifferentiated colonies in both treatment groups (24 colonies per group over 3 replicates) had by Day 7 grown to total of 51.1 and 79.5 mm2 (P < 0.001), and by Day 14 to 216.8 and 373.3 mm2 (P < 0.0001) in HIGH and LOW, respectively (see Table 1). In neither experiment were there differences in the differentiation status of the colonies between the treatments (mean 6% and 5% at Day 7, and 9% and 9% at Day 14 in HIGH and LOW, respectively). We conclude that culture in reduced oxygen improves growth of human embryonic stem cells. Table 1. Growth of hES colonies on high and low oxygen atmosphere (data from 3 replicates) We like to acknowledge Bresagen Ltd. for the supply of hesBGN-01 cell line.

scholarly journals Detailed method description for noninvasive monitoring of differentiation status of human embryonic stem cells

2014 ◽  
Vol 461 ◽  
pp. 60-66 ◽  
Author(s):  
Ellen Scheerlinck ◽  
Katleen Van Steendam ◽  
Mado Vandewoestyne ◽  
Trees Lepez ◽  
Veerle Gobin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Noninvasive Monitoring ◽  
Detailed Method ◽  
Differentiation Status

scholarly journals Lengthened G1 Phase Indicates Differentiation Status in Human Embryonic Stem Cells

2013 ◽  
Vol 22 (2) ◽  
pp. 279-295 ◽  
Author(s):  
Ashley Calder ◽  
Ivana Roth-Albin ◽  
Sonam Bhatia ◽  
Carlos Pilquil ◽  
Jong Hee Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
G1 Phase ◽  
Differentiation Status

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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