Rapid, Absolute, and Simultaneous Quantification of Specific Pathogenic Strain and Total Bacterial Cells Using an Ultrasensitive Dual-Color Flow Cytometer

2010 ◽  
Vol 82 (3) ◽  
pp. 1109-1116 ◽  
Author(s):  
Lingling Yang ◽  
Lina Wu ◽  
Shaobin Zhu ◽  
Yao Long ◽  
Wei Hang ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
Pathogenic Strain ◽  
Bacterial Cells ◽  
Color Flow ◽  
Simultaneous Quantification ◽  
Dual Color

scholarly journals Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland

2019 ◽  
Vol 475 ◽  
pp. 112348 ◽  
Author(s):  
Hana Glier ◽  
Ingmar Heijnen ◽  
Mathieu Hauwel ◽  
Jan Dirks ◽  
Stéphane Quarroz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Feasibility Study ◽  
Flow Cytometer ◽  
Color Flow ◽  
Clinical Laboratories

Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer

2008 ◽  
Vol 74B (2) ◽  
pp. 91-103 ◽  
Author(s):  
Sven Björnsson ◽  
Saga Wahlström ◽  
Eva Norström ◽  
Ingela Bernevi ◽  
Ulla O'Neill ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometer ◽  
Color Flow ◽  
Single Tube

scholarly journals Data-Driven Compensation for Flow Cytometry of Solid Tissues

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Nickolaas Maria van Rodijnen ◽  
Math Pieters ◽  
Sjack Hoop ◽  
Marius Nap
Keyword(s):  
Flow Cytometry ◽  
Propidium Iodide ◽  
Dna Content ◽  
Flow Cytometer ◽  
Data Driven ◽  
Color Flow ◽  
Operator Experience ◽  
Solid Tissues

Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phenotype flow cytometry acquisitions. The generated compensation values of the DDC algorithm are validated by comparison with manually determined compensation values. The results show that (1) compensation of two-color flow cytometry leads to comparable results using either manual compensation or the DDC method; (2) DDC can calculate sample-specific compensation trace lines; (3) the effects of two different approaches to calculate compensation values can be visualized within one sample. We conclude that the DDC algorithm contributes to the standardization of compensation for spectral cross-over in flow cytometry of solid tissues.

Simplification of Clinical Flow Cytometry by Adoption of Premixed Antibody Panels

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S112-S113
Author(s):  
J M Polski
Keyword(s):  
Flow Cytometry ◽  
Cost Effective ◽  
Standard Of Care ◽  
Flow Cytometer ◽  
Multiparameter Flow Cytometry ◽  
Complete Agreement ◽  
Color Flow ◽  
Additional Advantage ◽  
Medical Technologists ◽  
User Friendly

Abstract Introduction/Objective Multiparameter flow cytometry (MFC) is the standard of care for the diagnosis and monitoring of hematopoietic and lymphoid neoplasms. While MFC is a mature technology, new innovations are brought to the market. Such an innovation is the ClearLLab10C, FDA-cleared, 4-tube, 10-color, dry, premixed antibody panels (Beckman Coulter Diagnostics, Brea, California). Our laboratory decided to implement this new reagent system combined with 10-color clinical flow cytometer, Navios EX from the same vendor. This paper describes the clinical validation results for leukemia/lymphoma (LL) evaluation as well as logistical and labor savings in the laboratory. Methods Thirty specimens submitted for LL evaluations were tested by our existing 5-color flow cytometer with custom antibody combinations as well as by the Navios EX instrument with the ClearLLab10C product supplemented by additional antibody combinations for intracellular analysis. The validation cases included normal and abnormal cases, representing bone marrows, blood, lymph nodes, and a few other specimens. Results There was a complete agreement in the qualitative results. The new simplified premixed reagents allow for noticeable simplification of inventory and saving in labor. The existing LL antibody cocktails required stocking 41 antibodies. The new platform requires only 4 LL reagents for surface immunophenotyping and we elected to stock additional 8 antibodies for optional intracellular evaluation. The amount of manual pipetting is greatly reduced. One additional advantage of utilizing the new ClearLLab10C is the available electronic and book publication from the vendor illustrating typical results for 24 normal and abnormal specimens analyzed using ClearLLab10C. This resource together with a user-friendly analysis software Kaluza C greatly facilitate interpretation of 10-color results by pathologists, medical technologists, and especially by the pathologists in training. Conclusion ClearLLab10C product is a cost-effective and user-friendly solution for laboratories seeking a streamlined MFC and a great reduction of inventory and labor.

Immunophenotypes and Cytotoxic Functions of Lymphocytes in Patients with Hepatocellular Carcinoma

1997 ◽  
Vol 83 (4) ◽  
pp. 762-767 ◽  
Author(s):  
Sangeeta S. Chavan ◽  
Shubhada V. Chiplunkar
Keyword(s):  
Hepatocellular Carcinoma ◽  
Immune Responses ◽  
Peripheral Blood ◽  
Cytotoxic Lymphocytes ◽  
Healthy Individuals ◽  
Color Flow ◽  
Dual Color ◽  
Hla Dr ◽  
Tumor Tissues ◽  
Immunological Mechanisms

Aims and background Hepatocellular carcinoma (HCC) is one of the most common cancers in Asia. Immunological mechanisms are thought to play an important role in the control of tumor progression. The immune responses in HCC patients are poorly understood. In the present study, the proliferation and cytotoxic functions of lymphocytes from tumor tissues and peripheral blood of HCC patients were analysed. Simultaneously, the microcultures were phenotyped in order to determine the involvement of different lymphocyte subsets in mediating the cytotoxic function. Methods The frequencies of proliferating and cytotoxic lymphocytes from three tumor tissues and peripheral blood from ten HCC patients and nine healthy individuals were assessed by limiting dilution microculture analysis. These microcultures were phenotyped by single and dual color flow cytometry using monoclonal antibodies specific for CD4, CD8, CD56 and HLA-DR markers. Results The precursor frequencies of both proliferating and cytotoxic lymphocytes were found to be comparable in the peripheral blood of HCC patients and healthy individuals. Compared to peripheral blood, a marked reduction in the precursor frequencies of proliferating and cytotoxic lymphocytes was observed in the tumor tissues of HCC patients. In the tumor tissues, a significantly higher frequency of cytotoxic T cells compared to natural killer cells was observed. Dual color flow cytometric analysis revealed increased percentages of CD8+ HLA-DR+ lymphocytes compared to CD4+ HLA-DR+ cells in the tumor tissues. Conclusions Our results suggest that depressed immune responses at the tumor site might be responsible for the escape of tumor cells from the immune surveillance of the host.

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