scholarly journals Size-Sorting Combined with Improved Nanocapillary Liquid Chromatography−Mass Spectrometry for Identification of Intact Proteins up to 80 kDa

2010 ◽  
Vol 82 (4) ◽  
pp. 1234-1244 ◽  
Author(s):  
Adaikkalam Vellaichamy ◽  
John C. Tran ◽  
Adam D. Catherman ◽  
Ji Eun Lee ◽  
John F. Kellie ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Intact Proteins ◽  
Chromatography Mass Spectrometry ◽  
Size Sorting

A Laconic Review on Liquid Chromatography Mass Spectrometry (LC-MS) Based Proteomics Technology in Drug Discovery

2021 ◽  
pp. 194-201
Author(s):  
Shivani Sharma ◽  
Navdeep Singh ◽  
Amar Deep Ankalgi ◽  
Arti Rana ◽  
Mahendra Singh Ashawat
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
New Product Development ◽  
Pharmaceutical Products ◽  
Liquid Chromatography Mass Spectrometry ◽  
Intact Proteins ◽  
Chromatography Mass Spectrometry ◽  
The Future ◽  
Protein Components ◽  
The Stability

Liquid chromatography mass spectrometry is a powerful technique which is used for the new product development, manufacturing, and to the control the stability or drug abuse. These techniques were commonly preferred in pharmacokinetics studies in pharmaceutical products. The principle involved in the LC-MS technique was justified in our study to understand the basic fundamentals of LC-MS. Also, it is used in combination with HPLC for chemical analysis. Many applications and the future prospects have been highlighted regarding the use for LC-MS in analytical chemistry. It is basically depends on the metabolites which are collected in this technique, furthermore the chemicals are analyzed according to the nature of suitability of LC-MS. This technique was helpful in the analysis of protein components identified in terms of pharmacovigilance, organic/inorganic hybrid nanoflowering. Also, our study highlights the techniques involved in proteomics. In LC-MS-based proteomics, complex mixtures of proteins are first subjected to enzymatic cleavage, then the resulting peptide products are analyzed using a mass spectrometer; this is in contrast to "top-down" proteomics, which deals with intact proteins and is limited to single protein mixtures. So, this review aims to highlight the basic introduction, and principle involved in liquid chromatography-mass spectrometry (LC-MS). Also, the advantages, or application of LC-MS were studied. Most importantly the LC-MS based proteomics, and the future aspects of LC-MS technology were studied in this review.

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