Utility of Retention Prediction Model for Investigation of Peptide Separation Selectivity in Reversed-Phase Liquid Chromatography: Impact of Concentration of Trifluoroacetic Acid, Column Temperature, Gradient Slope and Type of Stationary Phase

2010 ◽  
Vol 82 (1) ◽  
pp. 265-275 ◽  
Author(s):  
Martin Gilar ◽  
Hongwei Xie ◽  
Aleksander Jaworski
Keyword(s):  
Liquid Chromatography ◽  
Temperature Gradient ◽  
Prediction Model ◽  
Trifluoroacetic Acid ◽  
Reversed Phase ◽  
Retention Prediction ◽  
Reversed Phase Liquid Chromatography ◽  
Column Temperature ◽  
Peptide Separation ◽  
Phase Liquid

Control of column temperature in reversed-phase liquid chromatography

2000 ◽  
Vol 869 (1-2) ◽  
pp. 211-230 ◽  
Author(s):  
R.G Wolcott ◽  
J.W Dolan ◽  
L.R Snyder ◽  
Stephen R Bakalyar ◽  
Megan A Arnold ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Column Temperature ◽  
Phase Liquid

Reversed-phase liquid chromatography and gas chromatography/mass fragmentography compared for determination of tricyclic antidepressant drugs.

1981 ◽  
Vol 27 (11) ◽  
pp. 1907-1911 ◽  
Author(s):  
D A Breutzmann ◽  
L D Bowers
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Tricyclic Antidepressants ◽  
Linear Regression Analysis ◽  
Antidepressant Drugs ◽  
Reversed Phase ◽  
Mass Fragmentography ◽  
Reversed Phase Liquid Chromatography ◽  
Column Temperature ◽  
Phase Liquid

Abstract We compare measurement of the tricyclic antidepressants desipramine, imipramine, nortryptaline, and amitryptaline by reversed-phase "high-performance" liquid chromatography and by gas chromatography/mass fragmentography. The liquid-chromatographic procedure does not require ion-pairing reagents, and involves detection at 215 nm. The within-day and between-day CVs for this method were less than 5% and less than 7%, respectively, and linear-regression analysis of peak height vs concentration for each of the drugs showed excellent correlation (r greater than 0.97) over the range 0-250 microgram/L. Potential interferences with the internal standard (N-desmethyldoxepin) and nortryptaline were oxazepam and N-desmethyldiazepam, respectively, as shown by mass spectrometry. The interference could be circumvented by increasing the column temperature to 50 degrees C. We conclude that reversed-phase liquid chromatography with detection in the far-ultraviolet wavelengths provides an alternative to gas-chromatographic procedures for the tricyclic antidepressants, provided the analyst has prior knowledge of the patient's other medications.

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