scholarly journals Ultrafast Differential Ion Mobility Spectrometry at Extreme Electric Fields Coupled to Mass Spectrometry

2009 ◽  
Vol 81 (19) ◽  
pp. 8048-8053 ◽  
Author(s):  
Alexandre A. Shvartsburg ◽  
Keqi Tang ◽  
Richard D. Smith ◽  
Martin Holden ◽  
Martyn Rush ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electric Fields ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Differential Ion Mobility Spectrometry

scholarly journals Ultrafast Differential Ion Mobility Spectrometry at Extreme Electric Fields in Multichannel Microchips

2009 ◽  
Vol 81 (15) ◽  
pp. 6489-6495 ◽  
Author(s):  
Alexandre A. Shvartsburg ◽  
Richard D. Smith ◽  
Ashley Wilks ◽  
Andrew Koehl ◽  
David Ruiz-Alonso ◽  
...  
Keyword(s):  
Electric Fields ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Differential Ion Mobility Spectrometry

scholarly journals Direct Measurement of Light and Heavy Antibody Chains Using Differential Ion Mobility Spectrometry and Middle-Down Mass Spectrometry

2019 ◽  
Author(s):  
Rafael D Melani ◽  
Kristina Srzentić ◽  
Vincent R Gerbasi ◽  
John P McGee ◽  
Romain Huguet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Asymmetric Waveform ◽  
Fast Fashion ◽  
Differential Ion Mobility Spectrometry ◽  
Ms Analysis ◽  
Polypeptide Sequence ◽  
Liquid Fractionation

AbstractThe analysis of monoclonal antibodies (mAbs) by a middle-down approach is a growing field that attracts the attention of many researchers and biopharma companies. Usually, liquid fractionation techniques are used to separate mAbs polypeptides chains before mass spectrometry (MS) analysis. Gas-phase fractionation techniques such as high-field asymmetric waveform ion mobility spectrometry (FAIMS) can replace liquid-based separations and reduce both analysis time and cost. Here, we present a rapid FAIMS tandem MS method capable of characterizing the polypeptide sequence of mAbs light (Lc) and heavy (Hc) chains in an unprecedented, easy, and fast fashion. This new method uses commercially available instruments and takes ∼ 24 minutes —40-60% faster than regular LC-MS/MS analysis — to acquire fragmentation data using different dissociation methods.

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