Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection

2009 ◽  
Vol 81 (17) ◽  
pp. 7468-7473 ◽  
Author(s):  
Josephine Ruta ◽  
Sandrine Perrier ◽  
Corinne Ravelet ◽  
Jennifer Fize ◽  
Eric Peyrin
Keyword(s):  
Small Molecule ◽  
Fluorescence Polarization ◽  
Small Molecule Detection

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

Small-Molecule Detection in Thiol–Yne Nanocomposites via Surface-Enhanced Raman Spectroscopy

2014 ◽  
Vol 86 (24) ◽  
pp. 12315-12320 ◽  
Author(s):  
Darryl A. Boyd ◽  
Francisco J. Bezares ◽  
Dennis B. Pacardo ◽  
Maraizu Ukaegbu ◽  
Charles Hosten ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Small Molecule ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Small Molecule Detection

Matrix-enhanced nanostructure initiator mass spectrometry (ME-NIMS) for small molecule detection and imaging

2016 ◽  
Vol 8 (46) ◽  
pp. 8234-8240 ◽  
Author(s):  
Tara N. Moening ◽  
Victoria L. Brown ◽  
Lin He
Keyword(s):  
Mass Spectrometry ◽  
Small Molecule ◽  
Tissue Imaging ◽  
Enhanced Sensitivity ◽  
Ms Imaging ◽  
Small Molecule Detection

ME-NIMS MS imaging (right): significantly enhanced sensitivity over conventional NIMS (left) in tissue imaging.

Fluorescence Polarization for the Evaluation of Small-Molecule Inhibitors of PCAF BRD/Tat-AcK50 Association

ChemMedChem ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. 928-931 ◽  
Author(s):  
Ping Hu ◽  
Xinghui Wang ◽  
Baiqun Zhang ◽  
Shuai Zhang ◽  
Qiang Wang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fluorescence Polarization ◽  
Small Molecule Inhibitors
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