Microfluidic Polyacrylamide Gel Electrophoresis with in Situ Immunoblotting for Native Protein Analysis

2009 ◽  
Vol 81 (19) ◽  
pp. 8177-8184 ◽  
Author(s):  
Mei He ◽  
Amy E. Herr
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein Analysis ◽  
Polyacrylamide Gel ◽  
Native Protein

scholarly journals Isolation of mouse myocardial gap junctions.

1980 ◽  
Vol 86 (3) ◽  
pp. 755-764 ◽  
Author(s):  
R W Kensler ◽  
D A Goodenough
Keyword(s):  
Gap Junctions ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Diffuse Band ◽  
Intercalated Disk

A new method is presented for the isolation of an enriched fraction of mouse myocardial gap junctions without the use of exogenous proteases. The junctions appear well preserved morphologically and similar to their appearance in situ. Contaminants of the preparation include fragments of the fascia adherens region of the intercalated disk. SDS polyacrylamide gel electrophoresis of the preparation reveals seven major bands with apparent mol wt of 28,000; 31,000; 33,500; 43,000; 47,000; 49,000; and 57,000. Only the bands at 38,000; 31,000; 33,500; and possibly the diffuse band at 47,000 copurify with the morphologically assayed gap junctions. Evidence is presented that the peptides at 43,000 and 57,000 are contained within the contaminating fascia adherens.

Detection of Antibacterial Polypeptide Activity in Situ after Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

2001 ◽  
Vol 299 (2) ◽  
pp. 274-276 ◽  
Author(s):  
Małgorzata Cytryńska ◽  
Agnieszka Zdybicka-Barabas ◽  
Piotr Jabłoński ◽  
Teresa Jakubowicz
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate
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