Quantitative Profiling of in Vivo Generated Cisplatin−DNA Adducts Using Different Isotope Dilution Strategies

2009 ◽  
Vol 81 (23) ◽  
pp. 9553-9560 ◽  
Author(s):  
D. García Sar ◽  
M. Montes-Bayón ◽  
E. Blanco González ◽  
L. M. Sierra ◽  
L. Aguado ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Isotope Dilution ◽  
Quantitative Profiling

scholarly journals Alkylated epidermal creatine kinase as a biomarker for sulfur mustard exposure: comparison to adducts of albumin and DNA in an in vivo rat study

2021 ◽  
Author(s):  
Dirk Steinritz ◽  
Robin Lüling ◽  
Markus Siegert ◽  
Julia Herbert ◽  
Harald Mückter ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Dna Adducts ◽  
Sulfur Mustard ◽  
Ex Vivo ◽  
Immunomagnetic Separation ◽  
Chemical Warfare Agent ◽  
Cysteine Residue ◽  
Islamic State ◽  
Rat Skin

AbstractSulfur mustard (SM) is a chemical warfare agent which use is banned under international law and that has been used recently in Northern Iraq and Syria by the so-called Islamic State. SM induces the alkylation of endogenous proteins like albumin and hemoglobin thus forming covalent adducts that are targeted by bioanalytical methods for the verification of systemic poisoning. We herein report a novel biomarker, namely creatine kinase (CK) B-type, suitable as a local biomarker for SM exposure on the skin. Human and rat skin were proven to contain CK B-type by Western blot analysis. Following exposure to SM ex vivo, the CK-adduct was extracted from homogenates by immunomagnetic separation and proteolyzed afterwards. The cysteine residue Cys282 was found to be alkylated by the SM-specific hydroxyethylthioethyl (HETE)-moiety detected as the biomarker tetrapeptide TC(-HETE)PS. A selective and sensitive micro liquid chromatography-electrospray ionization high-resolution tandem-mass spectrometry (µLC-ESI MS/HRMS) method was developed to monitor local CK-adducts in an in vivo study with rats percutaneously exposed to SM. CK-adduct formation was compared to already established DNA- and systemic albumin biomarkers. CK- and DNA-adducts were successfully detected in biopsies of exposed rat skin as well as albumin-adducts in plasma. Relative biomarker concentrations make the CK-adduct highly appropriate as a local dermal biomarker. In summary, CK or rather Cys282 in CK B-type was identified as a new, additional dermal target of local SM exposures. To our knowledge, it is also the first time that HETE-albumin adducts, and HETE-DNA adducts were monitored simultaneously in an in vivo animal study.

Characterization of DNA adducts of the carcinogen N-methyl-4-aminoazobenzene in vitro and in vivo

1980 ◽  
Vol 31 (1) ◽  
pp. 1-17 ◽  
Author(s):  
F.A. Beland ◽  
D.L. Tullis ◽  
F.F. Kadlubar ◽  
K.M. Straub ◽  
F.E. Evans
Keyword(s):  
Dna Adducts

The in vivo formation and repair of DNA adducts from 1′-hydroxysafrole

1981 ◽  
Vol 16 (1) ◽  
pp. 83-90 ◽  
Author(s):  
David H. Phillips ◽  
Philip C. Hanawalt ◽  
James A. Miller ◽  
Elizabeth C. Miller
Keyword(s):  
Dna Adducts

Effects of insulin on glucose turnover rates in vivo: Isotope dilution versus constant specific activity technique

Metabolism ◽  
1996 ◽  
Vol 45 (1) ◽  
pp. 82-91 ◽  
Author(s):  
Ole Hother-Nielsen ◽  
Jan Erik Henriksen ◽  
Jens Juul Holst ◽  
Henning Beck-Nielsen
Keyword(s):  
Isotope Dilution ◽  
Specific Activity ◽  
Glucose Turnover ◽  
Turnover Rates ◽  
Constant Specific Activity

Formation of DNA adducts by the food mutagen 2-amino-3,4,8-trimethyl-3H-imidazo [4,8-f]quinoxaline (4,8-DiMeIQx) in vitro and in vivo. Identification of a N-(2′-deoxyguanosin-8-yl)-4,8-DiMeIQx adduct

1994 ◽  
Vol 15 (11) ◽  
pp. 2553-2558 ◽  
Author(s):  
Henrik Frandsen ◽  
Spiros Grivas ◽  
Robert J. Turesky ◽  
Rolf Andersson ◽  
Lars O. Dragsted ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Food Mutagen

scholarly journals Repair of cis-platinum-DNA adducts by ABC excinuclease in vivo and in vitro.

1985 ◽  
Vol 163 (3) ◽  
pp. 817-823 ◽  
Author(s):  
I Husain ◽  
S G Chaney ◽  
A Sancar
Keyword(s):  
Dna Adducts

Effect of the Antioxidant Butylated Hydroxyanisole on In Vivo Formation of Benzo[a]pyrene Metabolite-DNA Adducts

1985 ◽  
pp. 241-251
Author(s):  
MARSHALL W. ANDERSON ◽  
PETER I. ADRIAENSSENS ◽  
CATHERINE M. WHITE ◽  
Y. M. IOANNOU ◽  
ALAN G. E. WILSON
Keyword(s):  
Dna Adducts ◽  
Butylated Hydroxyanisole

scholarly journals A human in vivo model for the determination of lead bioavailability using stable isotope dilution.

1996 ◽  
Vol 104 (2) ◽  
pp. 176-179 ◽  
Author(s):  
J H Graziano ◽  
C B Blum ◽  
N J Lolacono ◽  
V Slavkovich ◽  
W I Manton ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
In Vivo Model ◽  
Stable Isotope Dilution
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