A Noninvasive Thin Film Sensor for Monitoring Oxygen Tension during in Vitro Cell Culture

2009 ◽  
Vol 81 (22) ◽  
pp. 9239-9246 ◽  
Author(s):  
Peter C. Thomas ◽  
Michael Halter ◽  
Alessandro Tona ◽  
Srinivasa R. Raghavan ◽  
Anne L. Plant ◽  
...  
Keyword(s):  
Thin Film ◽  
Cell Culture ◽  
Oxygen Tension ◽  
Film Sensor ◽  
Thin Film Sensor ◽  
In Vitro Cell Culture

scholarly journals An In Vitro Cell Culture Model for Pyoverdine-Mediated Virulence

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Donghoon Kang ◽  
Natalia V. Kirienko
Keyword(s):  
Cell Culture ◽  
Virulence Factors ◽  
Model Organisms ◽  
Cell Culture Model ◽  
Chronic Infections ◽  
In Vitro Cell Culture ◽  
Mitochondrial Homeostasis ◽  
Culture Model ◽  
Wide Range

Pseudomonas aeruginosa is a multidrug-resistant, opportunistic pathogen that utilizes a wide-range of virulence factors to cause acute, life-threatening infections in immunocompromised patients, especially those in intensive care units. It also causes debilitating chronic infections that shorten lives and worsen the quality of life for cystic fibrosis patients. One of the key virulence factors in P. aeruginosa is the siderophore pyoverdine, which provides the pathogen with iron during infection, regulates the production of secreted toxins, and disrupts host iron and mitochondrial homeostasis. These roles have been characterized in model organisms such as Caenorhabditis elegans and mice. However, an intermediary system, using cell culture to investigate the activity of this siderophore has been absent. In this report, we describe such a system, using murine macrophages treated with pyoverdine. We demonstrate that pyoverdine-rich filtrates from P. aeruginosa exhibit substantial cytotoxicity, and that the inhibition of pyoverdine production (genetic or chemical) is sufficient to mitigate virulence. Furthermore, consistent with previous observations made in C. elegans, pyoverdine translocates into cells and disrupts host mitochondrial homeostasis. Most importantly, we observe a strong correlation between pyoverdine production and virulence in P. aeruginosa clinical isolates, confirming pyoverdine’s value as a promising target for therapeutic intervention. This in vitro cell culture model will allow rapid validation of pyoverdine antivirulents in a simple but physiologically relevant manner.

scholarly journals STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY

2018 ◽  
Vol 63 (1) ◽  
pp. 3795-3798
Author(s):  
Meifeng Chen ◽  
Xinying Ma ◽  
Xia Li ◽  
Mingjing Yin ◽  
Yanyun Li ◽  
...  
Keyword(s):  
Thin Film ◽  
Film Sensor ◽  
Thin Film Sensor ◽  
Vis Spectroscopy

scholarly journals Development of an in vitro cell culture model to study milk to plasma ratios of therapeutic drugs

2013 ◽  
Vol 45 (4) ◽  
pp. 325 ◽  
Author(s):  
Anurupa Maitra ◽  
Shahnaz Patel ◽  
VijayR Bhate ◽  
VilliS Toddywalla ◽  
MaithiliA Athavale
Keyword(s):  
Cell Culture ◽  
Cell Culture Model ◽  
In Vitro Cell Culture ◽  
Therapeutic Drugs ◽  
Culture Model

A simple and cost-effective zinc oxide thin film sensor for propane gas detection

2015 ◽  
Vol 157 ◽  
pp. 169-171 ◽  
Author(s):  
Vinoth Kumar Jayaraman ◽  
Arturo Maldonado Álvarez ◽  
María de la Luz Olvera Amador
Keyword(s):  
Thin Film ◽  
Zinc Oxide ◽  
Cost Effective ◽  
Gas Detection ◽  
Oxide Thin Film ◽  
Zinc Oxide Thin Film ◽  
Film Sensor ◽  
Thin Film Sensor
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