Direct Quantitation of the Quorum Sensing Signal, Autoinducer-2, in Clinically Relevant Samples by Liquid Chromatography−Tandem Mass Spectrometry

2009 ◽  
Vol 81 (15) ◽  
pp. 6374-6381 ◽  
Author(s):  
Shawn R. Campagna ◽  
Jessica R. Gooding ◽  
Amanda L. May
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quorum Sensing ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Autoinducer 2 ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

scholarly journals Identification of Quorum-Sensing Molecules of N-Acyl-Homoserine Lactone in Gluconacetobacter Strains by Liquid Chromatography-Tandem Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2694
Author(s):  
Ling-Pu Liu ◽  
Long-Hui Huang ◽  
Xiao-Tong Ding ◽  
Lin Yan ◽  
Shi-Ru Jia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quorum Sensing ◽  
Tandem Mass Spectrometry ◽  
Homoserine Lactone ◽  
Tandem Mass ◽  
Gram Negative ◽  
Sensing Systems ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Many Gram-negative bacteria can regulate gene expression in a cell density-dependent manner via quorum-sensing systems using N-acyl-homoserine lactones (AHLs), which are typical quorum-sensing signaling molecules, and thus modulate physiological characteristics. N-acyl-homoserine lactones are small chemical molecules produced at low concentrations by bacteria and are, therefore, difficult to detect. Here, a biosensor system method and liquid chromatography-tandem mass spectrometry were combined to detect and assay AHL production. As demonstrated by liquid chromatography-tandem mass spectrometry, Gluconacetobacter xylinus CGMCC No. 2955, a Gram-negative acetic acid-producing bacterium and a typical bacterial cellulose (BC) biosynthesis strain, produces six different AHLs, including N-acetyl-homoserine lactone, N-butanoyl-homoserine lactone, N-hexanoyl-homoserine lactone, N-3-oxo-decanoyl-homoserine lactone, N-dodecanoyl-homoserine lactone, and N-tetradecanoyl-homoserine lactone. Gluconacetobacter sp. strain SX-1, another Gram-negative acetic acid-producing bacterium, which can synthesize BC, produces seven different AHLs including N-acetyl-homoserine lactone, N-butanoyl-homoserine lactone, N-hexanoyl-homoserine lactone, N-3-oxo-octanoyl-homoserine lactone, N-decanoyl-homoserine lactone, N-dodecanoyl-homoserine lactone, and N-tetradecanoyl-homoserine lactone. These results lay the foundation for investigating the relationship between BC biosynthesis and quorum-sensing systems.

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