scholarly journals Femtomolar Concentration Detection Limit and Zeptomole Mass Detection Limit for Protein Separation by Capillary Isoelectric Focusing and Laser-Induced Fluorescence Detection

2009 ◽  
Vol 81 (5) ◽  
pp. 1741-1746 ◽  
Author(s):  
Lauren M. Ramsay ◽  
Jane A. Dickerson ◽  
Oluwatosin Dada ◽  
Norman J. Dovichi
Keyword(s):  
Detection Limit ◽  
Isoelectric Focusing ◽  
Fluorescence Detection ◽  
Protein Separation ◽  
Laser Induced Fluorescence ◽  
Capillary Isoelectric Focusing ◽  
Mass Detection ◽  
Laser Induced Fluorescence Detection ◽  
Concentration Detection Limit ◽  
Femtomolar Concentration

scholarly journals Single-photon laser-induced fluorescence detection of nitric oxide at sub-parts-per-trillion mixing ratios

2020 ◽  
Vol 13 (5) ◽  
pp. 2425-2439 ◽  
Author(s):  
Andrew W. Rollins ◽  
Pamela S. Rickly ◽  
Ru-Shan Gao ◽  
Thomas B. Ryerson ◽  
Steven S. Brown ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Fluorescence Detection ◽  
Narrow Band ◽  
Single Photon ◽  
Spectral Feature ◽  
Laser Induced Fluorescence ◽  
Mixing Ratios ◽  
Laser Induced Fluorescence Detection ◽  
Direct Measurements

Abstract. We describe a newly developed single-photon laser-induced fluorescence sensor for measurements of nitric oxide (NO) in the atmosphere. Rapid tuning of a narrow-band laser on and off of a rotationally resolved NO spectral feature near 215 nm and detection of the red-shifted fluorescence provides for interference-free direct measurements of NO with a detection limit of 1 part per trillion by volume (pptv) for 1 s of integration, or 0.3 pptv for 10 s of integration. Uncertainty in the sensitivity of the instrument is typically ±6–9 %, with no known interferences. Uncertainty in the zero of the detector is shown to be <0.2 pptv. The instrument was deployed on the NASA DC-8 aircraft during the NASA/NOAA FIREX-AQ experiment (Fire Influence on Regional to Global Environments Experiment – Air Quality) during July–September 2019 and provided more than 140 h of NO measurements over 22 flights, demonstrating the ability of this instrument to operate routinely and autonomously. Comparisons with a seasoned chemiluminescence sensor during FIREX-AQ in a variety of chemical environments provides validation and confidence in the accuracy of this technique.

Research of Laser Induced Fluorescence Detection Techniques Based on Microfluidic Devices

2014 ◽  
Vol 989-994 ◽  
pp. 2761-2763
Author(s):  
Yong Qiang Cheng ◽  
Cui Lian Guo ◽  
Bin Zhao ◽  
Li Yang
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Recent Progress ◽  
Biochemical Analysis ◽  
Detection Method ◽  
Microfluidic Devices ◽  
Laser Induced Fluorescence ◽  
Detection Techniques ◽  
Laser Induced Fluorescence Detection ◽  
Optical Structure

Laser induced fluorescence (LIF) detection technique is one of the most sensitive detection method in micro total analytical systems (μTAS) as its good monochromaticity, strong collimation and high optical density. It is widely used in biochemical analysis systems and the detection limit could reach 10-9-10-12mol/L. Due to the important role in μTAS, different optical structure arrangements of LIF detection techniques were continuously developed. We reviewed the recent progress of the optical structure arrangement and the applications of the LIF detection techniques.

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