Regenerable Tethered Bilayer Lipid Membrane Arrays for Multiplexed Label-Free Analysis of Lipid−Protein Interactions on Poly(dimethylsiloxane) Microchips Using SPR Imaging

2009 ◽  
Vol 81 (3) ◽  
pp. 1146-1153 ◽  
Author(s):  
Joseph D. Taylor ◽  
Matthew J. Linman ◽  
Thomas Wilkop ◽  
Quan Cheng
Keyword(s):  
Protein Interactions ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Label Free ◽  
Bilayer Lipid ◽  
Spr Imaging ◽  
Lipid Protein Interactions

Microfluidic Emulsion Generation and Trapping Enabling Droplet-Interfaced Bilayer Lipid Membrane Arrays

2009 ◽  
Author(s):  
C. Shao ◽  
D. L. DeVoe
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Protein Interactions ◽  
Lipid Membranes ◽  
High Throughput Screening ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Single Molecule Level ◽  
Bilayer Lipid ◽  
Ion Translocation

Freestanding bilayer lipid membranes provide an exceptional platform for measurements of lipid/protein interactions and ion translocation events at the single molecule level. For drug screening applications, large arrays of individual bilayer supports are required. However, an effective method for generating, stabilizing, and monitoring arrays of lipid bilayers remains elusive. Here we investigate a novel approach towards the facile generation of bilayer arrays for high throughput screening. The approach takes advantage of fundamental microfluidic capabilities by combining an emulsion generator with droplet-interfaced membrane formation, allowing for fully-automated production of membrane arrays whose density is, in principle, unlimited.

Capacitance extraction method for a free-standing bilayer lipid membrane formed over an aperture in a nanofabricated silicon chip

2020 ◽  
Vol 59 (SI) ◽  
pp. SIIK02
Author(s):  
Yasutaka Tomioka ◽  
Shogo Takashima ◽  
Masataka Moriya ◽  
Hiroshi Shimada ◽  
Fumihiko Hirose ◽  
...  
Keyword(s):  
Extraction Method ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Free Standing ◽  
Capacitance Extraction ◽  
Bilayer Lipid

Concentration and time dependant behavior of chlorpromazine interaction with supported bilayer lipid membrane

2006 ◽  
Vol 51 (12) ◽  
pp. 2512-2517 ◽  
Author(s):  
Xiaohua Liu ◽  
Haixin Bai ◽  
Weimin Huang ◽  
Liangwei Du ◽  
Xiurong Yang ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Bilayer Lipid ◽  
Supported Bilayer
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