Virtual Quantification of Metabolites by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry: Predicting Ionization Efficiency Without Chemical Standards

2009 ◽  
Vol 81 (7) ◽  
pp. 2506-2515 ◽  
Author(s):  
Kenneth R. Chalcraft ◽  
Richard Lee ◽  
Casandra Mills ◽  
Philip Britz-McKibbin
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Efficiency ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass

scholarly journals Analysis of Inositol Phosphate Metabolism by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS)

2020 ◽  
Author(s):  
Danye Qiu ◽  
Miranda S. Wilson ◽  
Verena B. Eisenbeis ◽  
Robert K. Harmel ◽  
Esther Riemer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Inositol Phosphate ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Phosphate Metabolism ◽  
Esi Ms ◽  
Inositol Phosphate Metabolism

AbstractThe analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is highly desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables for the first time the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [13C6]-myo-inositol or [13C6]-D-glucose. In doing so, we uncover that there must be unknown inositol synthesis pathways in mammals, highlighting the unique potential of this method to dissect inositol phosphate metabolism and signalling.

Capillary electrophoresis-laser induced fluorescence-electrospray ionization-mass spectrometry: A case study

2005 ◽  
Vol 26 (7-8) ◽  
pp. 1389-1397 ◽  
Author(s):  
Carolin Huhn ◽  
Christian Neusüß ◽  
Matthias Pelzing ◽  
Ute Pyell ◽  
Joachim Mannhardt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Laser Induced Fluorescence ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass
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